迷走神经刺激建立迷走神经介导的急性房颤犬模型

Cervical vagua trunck stimulation for establishing paroxysmal atrial fibrillation model on canine

目的探讨建立迷走神经张力增高介导的急性房颤(atrial fibrillation,AF)动物模型的方法。方法成年杂种犬16只,分离暴露颈部双侧迷走神经干,植入刺激电极,分为3组:对照组(control组,n=4),仅行高右房刺激600次/min刺激。右侧迷走神经干刺激组(RVT组,n=6),行高右房刺激+右侧迷走神经干刺激。左侧迷走神经干刺激组((LVT组,n=6),行高右房刺激+左侧迷走神经干刺激。以1 000次/min、不同电压刺激强度行左右迷走神经干刺激,测定心率、AF诱发率。结果对照组行猝发刺激(S1S1刺激)时,AF的诱发率为47.62%,高于(S1S2刺激)的AF诱发率(9.52%)(P<0.05)。RVT组S1S1刺激AF的诱发率为85.71%,高于S1S2刺激的AF诱发率(26.98%)(P<0.05)。LVT组S1S1刺激AF的诱发率为64.29%,高于S1S2刺激的AF诱发率(19.05%)(P<0.05)。结论单侧迷走神经刺激+快速右心房起搏可成功建立迷走神经介导的阵发性AF犬模型。右侧迷走神经干刺激AF的诱发率高于左侧诱发率。

Objective To establish atrial fibrillation （AF） model methodology by stimulating cervical vagua trunck on canine. Methods Bilateral cervical vago-sympathetic nerves from sixteen mongrel dogs were disassociated,exposed and stimulated. The dogs were divided into 3 groups, control group （n =4） which underwent rapid atial pacing （600 times/min） only. Right vagua trunck （RVT） group （n =6） which undertook high right atrial stimulation＋right vagus nerve stimulation and Left vagua trunck （LVT） group （n = 6） which undetook high right atrial stimulation＋the left vagus nerve stimulation. The heart rate and AF induction rate were measured in different stimulation intensity. Results The effect on AF inducibility. In control group, the induction rate of AF was significantly increased with S1S1 stimulation （47.62 %） com pared with S1S2 （9.52%） （P %0.05）. In RVT group, the induction rate of AF was significantly increased with S1S1 stimulation （85.71%） compared with S1 82 （26. 98 %） （P d0.05）. In LVT group, the induction rate of AF was significantly increased with S1S1 stimulation （64.29%） compared with S1S2 （19.05%） （P %0.05）. Conclusion Unilateral cervical vagua trunck stimulation plus rapid atial pacing can successfully establish canine model of acute AF mediated by vagus nerve, vagus nerve stimulation significantly increase AF induction rate. The AF inducibility was higher by RVT than LVT stimulation.