HPV16 E6/E7融合基因真核表达载体的构建

Eukaryotic expression vector construction of human papillomavirus type 16 E6/E7 fusion gene

目的构建pEGFP-N1-E6/E7融合基因真核表达载体。方法应用聚合酶链反应技术(polymerasechain reaction,PCR)从SiHa细胞中扩增出人类乳头瘤病毒16型(HPV16)E6/E7基因全长片段,并将其克隆到真核表达载体pEGFP-N1上。结果获得了包含全长阅读框架的HPV16E6/E7基因,并成功克隆到真核表达载体中。结论成功构建了pEGFP-N1-E6/E7融合基因真核表达载体。

Objective To construct pEGFP-N1-E6/E7 fusion gene into eukaryotic expression vector. Methods PCR was used to amplify the fragment that contained HPV16 E6/E70RF from SiHa cells. The amplified fragment was linked with eukaryotic expression vector pEGFP-N1. Resuts DNA fragment that contained E6/E70RF was gained, and we got a construct of pEGFP-N1-E6ET. Conclusion Eukaryotic expression vector of E6/E7 fusion gene of HPV16 was constructed successfully.