摘要
目的:建立HPLC测定金荞麦胶囊中原儿茶酸含量的方法。方法:采用反相高效液相色谱法,C18柱,流动相为甲醇-0.3%冰醋酸溶液梯度洗脱,检测波长为255 nm,流量1.0 mL.min-1,柱温30℃。结果:原儿茶酸浓度在0.016 58～0.331 6μg范围内线性关系良好(r=0.999 9),测定平均回收率为97.5%(n=6),RSD为1.1%。结论:所建方法准确、快速,可作为金荞麦胶囊中原儿茶酸的含量测定方法。
Objective： To establish an HPLC method for determination of protocatechuic acid in Jinqiaomai Capsules. Method： The HPLC method was carried on C18 column using methanol-0. 3% glacial acetic acid as the mobile phase to gradient elution,and the detection wavelength was 255 nm, the flow rate was 1.0 mL · min - L and the temperature of column was 30 ℃. Result： In the HPLC method,the calibration curve for protocateehuic acid was linear in the range of 0. 016 58-0. 331 6 ug （r =0. 999 9）. The average recovery for protocatechuie acid was 97.5 % , RSD was 1.1% （ n = 6 ）. Conclusion ： The method is convenient, precise and reliable for determining the content of protoeatechuie acid in Jinqiaomai Capsules.
出处
《中国药品标准》
CAS
2012年第6期422-424,共3页
Drug Standards of China
