RP-HPLC同时测定复方阿司匹林搽剂中三组分的含量

Determination of Three Constituents in Compound Aspirin Liniment by RP-HPLC

目的:测定复方阿司匹林搽剂中阿司匹林、苯甲酸和水杨酸的含量。方法:使用ZORBAX Eclipse XDB-C18(4.6 mm×150 mm,5μm),流动相为乙腈-甲醇-0.01 mol.L-1磷酸二氢钾溶液-三乙胺(5∶40∶55∶0.1)(用磷酸调pH值至2.5),柱温30℃,流量1.0 mL.min-1,检测波长277 nm。结果:阿司匹林、苯甲酸和水杨酸的线性范围分别为:40.32～403.20μg.mL-1(r=0.999 8),40.66～406.56μg.mL-1(r=0.999 8),38.58～385.76μg.mL-1(r=0.999 8);平均回收率分别为99.7%、99.8%、100.7%;RSD分别为0.92%、0.59%、0.46%。结论:该方法简便、灵敏、分离度好,其他成分无干扰,适用于控制复方阿司匹林搽剂产品质量。

Objective： To establish a method for the determination of aspirin, benzoic acid and salicylic acid in compound aspirin liniment. Methods： The RP-HPLC was performed on a ZORBAX Eclipse XDB-C18 column （4. 6 mm × 150 mm, 5 um）, with acetoni- trile-methanol-0. 01 mol · L-1 KH2PO4 solution-triethylamine （5：40：55： 0. 1 ） （adjusted to pH 2. 5 with phosphoric acid） as the mo- bile phase and the column temperature was 30 ℃. The flow rate was 1, 0 mL · min- 1 and the detection wavelength was 277 nm. Results： The linear ranges of aspirin, benzoic acid and salicylic acid were 40. 32-403.20 ug · mL-1 （r =0. 999 8） ,40. 66-406. 56 ug · mL-1 （r =0. 999 8） ; 38.58-385.76 ug · mL-1 （r =0. 999 8） respectively. The average recoveries were 99.7% （ RSD =0. 92% ）, 99. 8% （RSD = 0. 59% ） and 100. 7% （RSD = 0. 46% ） respectively. Conclusion： The method is simple, sensitive, accurate and suitable for the quality control of Compound Aspirin Liniment.