表没食子儿茶素没食子酸醋对人胃癌细胞迁移、袭和FoxM1基因表达的影响

Effects of EGCG on migration and invasion and FoxM1 expression of human gastric cancer cell

目的观察表没食子儿茶素没食子酸醋(表没食子儿茶素没食子酸醋,EGCG)对人胃癌细胞迁移、侵袭的影响,并探讨其可能机制。方法采用不同浓度的EGCG处理人胃癌BGC823细胞后,以划痕试验方法检测癌细胞迁移能力,以Transwell方法检测癌细胞侵袭能力,采用蛋白质印迹方法检测FoxM1基因蛋白水平变化。结果人胃癌BGC823细胞经EGCG处理后,迁移和侵袭能力均明显下降,且呈剂量依赖性(P<0.01)。EGCG处理组FoxM1基因蛋白水平明显下调,且呈浓度依赖性。结论 EGCG可降低人胃癌细胞侵袭能力,下调FoxM1基因表达,是其重要机制之一。

Objective To study the effects and mechanism of EGCG on migration and invasion of human gastric cancer cell.Methods After human gastric cancer BGC823 cell were treated with different doses of EGCG, and migration of cancer cell was studied by wound scratch assay,and invasion ability were determined by Transwell assay,and the e protein of MK gene were detected by Western Blot assay.Results EGCG could significantly inhibit both invasion ability and a migration in a dose-dependent manner.Conclusion EGCG could inhibit migration and invasion of gastric carcinoma cell line BGC823 through down-regulating of FoxM1 expression.