反相高效液相色谱法测定血浆中硫普罗宁钠

RP-HPLC Determination of Sodium Tiopronin in Plasma

采用反相高效液相色谱法对血浆中硫普罗宁钠进行测定。血浆样品经乙腈提取,所得提取液再经40℃氮气吹干,残留物经10mg.L-1亚硫酸氢钠溶液溶解后,用Agilent C18色谱柱(250mm×4.6mm,5μm)分离,以pH 2.5乙腈-水(8+92)溶液为流动相洗脱,用紫外检测器于波长203nm处测定。硫普罗宁钠的质量浓度在2.5～1 000mg.L-1范围内与其峰面积呈线性关系,测定下限(10S/N)为0.65mg.L-1。方法用于血浆样品分析,测得回收率在102%～107%之间,日内和日间相对标准偏差(n=6)均小于10%。

A method of RP HPI.C for the determination of sodium tiopronin in plasma was proposed. Sample of plasma was extracted with acetonitrile and the eluate obtained was ewtporated to dryness by N2-blowing at 40 ℃. The residue was dissolved with 10 mg · L^-1 NaHSO3 solution and separated by using Agilent C18 column （250 mm×4.6 mm, 5 μm） as stationary phase and a mixture of acetonitrile-H2O （8＋92） of pH 2. 5 as mobile phase, and the eluate from the colunm was used for HPLC analysis with UV detector at the wavelength of 203 nm. Linear relationship between values of peak area and mass concentration of sodium tiopronin was kept in the range of 2.5 to 1 000 mg · L ^-1, with lower limit of determination （10S/N） of 0. 65 mg · L ^-1 The proposed method was applied to the analysis of sample of plasma, giving values of recovery in the range of 102%- 107%. Values of intraday and interday relative standard deviation （n=6） were found to be less than 10%.