促红细胞生成素抑制正常及缺铁性贫血大鼠铁调素的表达

Inhibitory Effect of Erythropoietin on Hepcidin Expression in Normal and IDA Rats

目的:探讨促红细胞生成素(EPO)在缺铁性贫血(IDA)大鼠模型中对铁调素(hepcidin)表达的影响。方法:通过反复放血和饲喂低铁饲料,建立IDA大鼠动物模型,比较药物干预组(EPO)与非干预组及正常对照组与正常给药组(EPO)大鼠血清hepcidin表达的差异。Western blot检测肝脏内信号因子CCAAT/增强子结合蛋白α(C/EBPα)含量。结果:IDA模型组腹腔注射EPO后,血清hepcidin和肝脏C/EBPα含量显著降低。EPO对正常大鼠hepcidin表达也有显著抑制作用(P<0.05)。结论:hepcidin可能参与了IDA的发生,在正常大鼠和IDA大鼠中,EPO能通过下调C/EBPα阻碍其激活hepcidin启动子最终抑制hepcidin的表达。

Objective：To investigate the effect of eiythropoietin（EPO） on the serum hepcidin expression in iron deficiency anemia （IDA） rats.Method：IDA rat model was established by repeated bloodletting and low-iron diet feeding.The hepcidin expression in normal rats with or without EPO intervention and IDA rats with or without EPO intervention was respectively detected and compared. Result：After the intra-peritoneal injection of EPO,hepcidin expression and C/EBPa levels decreased in the IDA group.EPO also significantly inhibited hepcidin expression in the normal rats（P0.05）.Conclusion：Hepcidin plays an important role in the pathogenesis of IDA.Both in normal and IDA rats,EPO can decrease hepcidin expression levels by declining C/EBPa to hinder the activation of the hepcidin promoter.