扁担藤总黄酮和总三萜提取工艺及抗氧化研究

Extraction Technology and Antioxidant Activity of Total Flavonoids and Total Triterpenoids from Tetrastigma planicaule

目的:研究扁担藤总黄酮和总三萜的提取工艺及抗氧化活性。方法:采用正交试验优化扁担藤中总黄酮和总三萜的最佳提取工艺,并采用FRAP法、水杨酸法和ABTS法测定其抗氧化能力。结果:扁担藤总黄酮提取的最佳条件为70%乙醇在70℃超声波提取3次,每次1 h;总三萜提取的最佳条件为60%乙醇在70℃微波提取2次,每次5 min。与对照品抗坏血酸和茶多酚相比较,富含黄酮的扁担藤的Fe3+还原抗氧化能力和清除ABTS+.能力均大于茶多酚,但清除OH.的能力均小于二者。结论:超声法提取总黄酮和微波法提取总三萜效果最好,扁担藤具有一定的抗氧化性能。

Objective： To study the optimum extraction technology of total flavonoids and total triterpenoids from Tetrastigma planicaule and their antioxidant activity.Methods：Optimized the extraction of total flavonoids and total triterpenoids using an L9（34） orthogonal array design,and the antioxidant activity was extimated by FRAP assay,salicylicl acid assay and ABTS assay.Results：The best extraction conditions for total flavonoids from Tetrastigma planicaule were as follows：70 ℃ of 70% ethanol ultrasound-assisted extracting for 1 h and extracting three times,and total triterpenoids was：70 ℃ of 60% ethanol microwave extracting for 5 min and extracting two times.Compared with the positive control samples VC and tea polyphenol,the activity of reducing Fe3＋ antioxidant and scavenging ABTS＋· of flavonoids from Tetrastigma planicaule was higher than that of tea polyphenol,but scavenging OH· was lower than theirs;Conclusion：The method of using ultrasound-assisted extraction to extract total flavonoids and microwave extraction to extract total triterpenoids is the best,the extracts of Tetrastigma planicaule also shows certain antioxidative activity.