摘要
为探讨弱筋小麦籽粒淀粉合成对花后高温的响应机制,以弱筋小麦扬麦15为试验材料,研究花后不同时期35℃高温处理对籽粒淀粉积累、淀粉合成酶(AGPase、GBSS、SSS、SBE)活性以及淀粉合成酶基因(AGPase1、GBSSI、SSSIII、SBEI)表达的影响。结果表明:小麦花后不同时期经35℃高温处理后,其籽粒淀粉积累量、淀粉合成酶活性以及淀粉合成酶基因相对表达量均呈现下降趋势,各处理下降的幅度均表现为:花后5~7d>花后10~12d>花后15~17d>花后20~22d>花后25~27d,且花后5~7d高温处理下降的幅度最大。4种淀粉合成酶基因中,GBSSI和GBSS对温度最为钝感,SSSIII和SSS对温度最为敏感。
The grain of Yangmail5 was employed to analysis the responses of starch synthase gene ( AGPasel, GBSSI, SSSIII, SBE1)expressions, the activities of starch synthases (AGPase, GBSS, SSS, SBE) , and starch accumulation of grain heat-treated at the level of 35℃ during different growth stage after anthesis. Results showed that the decrements of these parameters among different high-temperature treating periods followed the order of 5 -7 DAA 〉 10 -12 DAA 〉 15 -17 DAA 〉 20 -22 DAA 〉 25 - 27 DAA. The inhibitive effects were more significant under 5 -7 DAA. GBSSI and GBSS were insensitive to high temperatures, while SSSIII and SSS were sensitive to high temperatures.
出处
《核农学报》
CAS
CSCD
北大核心
2012年第9期1311-1316,共6页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金资助项目(31071340)
江苏省"六大人才高峰"项目(07-G-008)
扬州大学自然科学基金项目(2007cxj015)
江苏高校省级重点实验室开放课题(K10006)
关键词
小麦
花后高温
淀粉积累
淀粉合成酶活性
淀粉合成酶基因表达
wheat
high temperature after anthesis
starch accumulation
the activities of starch synthases
the expression levels of starch synthase genes