芒针透刺对脊髓损伤后脊髓诱发电位影响的实验研究

To discuss the effects of point-through-point acupuncture with elongated needle to SCEP for spinal cord injury by animal experiment

目的:探讨芒针透刺对脊髓损伤后脊髓诱发电位影响及其机理。方法:采用日本大耳兔以改良式ALLEN’S脊髓损伤法造模,将其分为模型组、芒针透刺组、假手术组,每组又分5小组:术后即刻组、术后3 d组、术后7 d组、术后14 d组、术后28 d组,以Tarlov评分、脊髓诱发电位之P2潜伏时和波幅为观察指标,观察芒针透刺秩边-水道穴对下肢肌力感觉和脊髓诱发电位的影响。结果:兔脊髓损伤后均出现下肢瘫痪,经治疗5 d后芒针组较模型组tarlov评分明显改善(P<0.01);经芒针治疗4周后P2波幅较术后初期明显增宽、增大,而4周后P2潜伏期较术后初期明显缩短,基本恢复至术前水平,两者与模型组比较均有统计学意义(P<0.05)。结论:芒针透刺能改善下肢瘫痪,促进下肢肌力恢复,加快脊髓诱发电位波幅和延迟时间的恢复,其机理可能与修复脊髓神经细胞及其神经信号传导通路,阻断了神经细胞及其神经传导通路的进一步损伤等有关。

Objective：To discuss the effects of point-through-point acupuncture with elongated needle to SCEP for spinal cord injury （SPI） by animal experiment. Methods：Japanese rabbits taken as SPI model by modified ALLEN＇ S method were divided into 3 groups-blank group,model group,elongated needle acupuncture group. Every group was further divided into 5 groups：shortly after surgery,3 days after surgery,7 days after surgery, 14 days after surgery,28 days after surgery. The indicators including the Tarlov score,incubate time of P2 and its volume in SCEP were observed. The indexes could indicate the relationship of acupuncture （ aeupoints ：Zhibian-Shuidao） and myo-dynamia, SCEP as well. Results：All Japanese rabbits had lower limbs paralysis after spinal cord injury,after 5 days point-through-point acu-puncture the Tarlov scores in elongated needle acupuncture group were improved compared with that in model group （P 〈 0.01 ） ;their P2 waves after 4 weeks of treatment were wider and more magnified than that right after the surgery, the P2 incubation period reduced to the level before surgery, the differences were significant compared to model group （P 〈 0.05 ）. Conclusion ：The point-through-point acupuncture with elongated needle is effective for the lower limb paralysis, it could accelerate the recovery of the myodynamia and increase the amplitude and incubate time of SCEP. The mechanism is properly related with helping the reparation of neurons in spinal cord and the signal transduc- tion pathways, blocking-up of their further damage.