益骨口服液对去势大鼠骨质疏松症外周血清炎性因子的影响

Effects of Yigu oral liquid on the peripheral Serum Inflammatory factor in ovariectomized rats

目的:观察益骨口服液对去势大鼠骨质疏松症外周血清炎性因子的影响。方法:将80只SD大鼠随机分4组,第1组(益骨口服液组)、第2组(密盖息组)、第3组(益骨口服液+密盖息组)、第4组(生理盐水对照组),每组各20只;造模后,分别予益骨口服液、密盖息、益骨口服液+密盖息、生理盐水等不同药物灌胃,运用ELISA技术于治疗后第10天、第30天、第60天、第90天四个时点测定血清炎症因子COX-2、PGE2、cAMP的含量。结果:数据经过重复测量的方差分析,均存在分组效应(P=0.000、P=0.000、P=0.000)和时间效应(P=0.000、P=0.000、P=0.000),第1组在不同时间点对血清COX-2、PGE2、cAMP含量的比较,差异有统计学意义(P=0.026、P=0.039、P=0.031),在治疗后第10天,第1组与第4组两组间,差异无统计学意义(P=0.075、P=0.053、P=0.056),在治疗后第30天,第1组与第4组两组间比较,差异有统计学意义(P=0.035、P=0.043、P=0.021),在第60、90天,两组间比较,差异有统计学意义(P=0.000、P=0.000、P=0.000),在第10天、30天、60天、90天,第1组与第2、3组比较均无统计学意义(P>0.05)。结论:益骨口服液能降低去势大鼠外周血清中COX-2和PGE2,cAMP的含量,对去势大鼠骨质疏松疼痛具有一定抑制作用,在一定程度上,这是其治疗骨质疏松疼痛的作用机制之一。

Objective：To observe the effect of Yigu oral liquid by detecting peripheral serum Inflammatory factor in ovariectomized rats. Methods ： Eighty Female Wistar rats were randomly divided into 4 groups, group 1 （ Yigu oral liquid group ） , group 2 （ calcitonin group）, group 3 （Yigu oral liquid group ＋ calcitonin group）, group 4 （saline control group）, The ovariectomized rats were excised in the groups, rats in the group1 were administed with Yigu oral liquid; rats in the group 2 were administed with calcitonin; rats in the group 3 were administed with Yigu oral liquid group ＋ calcitonin ; rats in the group 4 were administed with saline. The rats were killed after treat-ment, and the peripheral Serum Inflammatory factors of COX-2 ,PGE2 ,cAMP were detected at the 10th day ,30th day ,60th day ,90th day re- spectively. Results：Repeated Measures Analysis of Variance （ANOVA）was used in analyzing the contents of COX-2, PGE2, cAMP in the four groups The analytic result displayed that there were group effect （ P = 0. 000, P = 0. 000, P = 0. 000） and time effect （ P = 0. 000, P = 0. 000, P = 0.000 ）. The contents of the group 1 were compared at four different times ： there was statistical difference （ P = 0. 026, P = 0. 039 ,P = 0.031 ）. At the 10th day after treatment, there was no statistical difference between the group 1 and group 4 （ P = 0. 075 ,P = 0. 053 ,P = 0. 056 ） , at the 30th day after treatment, there was statistical difference between the group 1 and group 4 （ P = 0. 035 ,P = 0. 043 P =0. 021 ） , at the 60th day and 90th day after treatment ,there was significant statistical difference between the group 1 and group 4（P = 0. 000 ,P = 0. 000 ,P = 0. 000）. At the 10th day ,30th day ,60th day ,90th day respectively, there was no statistical difference among group 1, group 2 and group 3 （ P 〉 0.05 ）. Conclusion ： Yigu oral liquid can reduce content of COX-2 and PGE2, cAMP in the peripheral serum of the ovariectomized rats, in some degree, the osteoporosis pain could be inhibited, and there may be an important therapeutic mechanism in osteoporosis pain.