2010～2011年乌鲁木齐地区住院呼吸道感染患儿人博卡病毒检测与分析

Detection and analysis of human bocavirus in children hospitalized with respiratory tract infection from 2010 to 2011 in Urumqi area

目的了解新疆乌鲁木齐地区住院呼吸道感染患儿中人博卡病毒（humanbocavirus，HBoV）的检出情况。方法收集新疆维吾尔自治区人民医院2010年1月至2011年12月住院呼吸道感染患儿鼻咽抽吸物标本525例，用巢氏PCR扩增人HBoV NS1片段检测HBoV1～4型。结果在525份标本中，HBoV总阳性检出率为8．38％（44／525），其中HBoV1型6例，HBoV2型38例，未检出HBoV3、4型。各民族患儿之间HBoV阳性检出率统计分析发现，维族、回族患儿分别高于汉族患儿，差异有统计学意义（P〈0．05）。HBoV1型与参考株核苷酸序列相似度为97．7％～99．4％，HBoV2型与参考株核苷酸序列相似度为93．6～100％。HBoV的检出时间主要以冬春季为主，HBoV感染在年龄及性别方面差异均无统计学意义。结论2010～2011年乌鲁木齐地区呼吸道感染的住院患儿存在HBoV1、2型的流行，以HBoV2型为主，维族、回族患儿HBoV的检出率分别高于汉族患儿。

Objective To study human bocavirus （HBoV） infections in hospitalized children with respiratory tract infection in Urumqi area. Methods Nasopharyngeal aspirates （NPA） samples were collected from 525 hospitalized children with respiratory tract infection at People＇s Hospital of Xinjiang Uygur Autonomous Region from 2010 to 2011. Nested PCR was used to amplify NS1 fragments to screen HBoV 1 to HBoV 4. Results The overall frequency of HBoVs was 8.38% （44/525）, including six cases of HBoV 1, thirty-eight cases of HBoV 2. HBoV 3 and HBoV 4 were not detected. HBoVs positive rates of Uygur and ldui children were statistically significantly higher than Han respectively （P 〈 0. 05 ）. Nucleotide sequences of six strains of HBoV 1 were 97. 7% -99.4% identical with that of 3 reference strains. Thirty-eight strains of HBoV 2 had 93.6 ~ 100% identical nucleotide sequence with 3 reference strains. HBoVs mainly occurred in winter and spring. No statistically significant differences in sex and ages were found. Conclusions HBoV l and HBoV 2 were circulating among hospitalized children with respiratory tract infection from 2010 to 2011 in Urumqi area with HBoV 2 as the dominant subtype. HBoVs positive rates of Uygur and Hui children were higher than Han respectively.