诱生型一氧化氮合酶在胆道感染大鼠肝细胞中的表达

Expression of inducible nitric oxide synthase in hepatocytes of rats with biliary tract infection

目的 了解诱生型一氧化氮合酶 (induciblenitricoxidesynthase ,iNOS)在胆道感染大鼠肝细胞中表达的情况及其规律。方法 制作大鼠胆道感染模型 ,采用还原型辅酶Ⅱ黄递酶组织化学法检测大鼠肝细胞中iNOS的表达。结果 大鼠胆道感染 2h后肝细胞即有iNOS的表达切片积分光度(13 5 8± 0 6 4) ,与对照组切片积分光度 (3 5 9± 0 2 8)相比 ,P <0 0 1。 8h达到峰值切片积分光度(2 9 2 7± 0 90 ) ,2 4h至 48h仍有较高表达切片积分光度分别为 (19 47± 0 6 5 )和 (19 96± 0 78)。结论 胆道感染时肝细胞可持续高效地表达iNOS ,提示胆道感染时肝脏是合成NO的重要器官 。

Objective To study the regular pattern of expression of inducible nitric oxide synthase (iNOS) in hepatocytes of rats with biliary tract infection. Methods A biliary tract infection model was made in rats and NADPH diaphorase staining was used to determine the expression of iNOS in hepatocytes of rats. Results iNOS was expressed in rat hepatocytes as early as 2 hours after biliary tract infection. The integral luminosity of sections was (13.58±0.64), which was much higher than that of the controls (3.59±0.28) (P<0.01). It reached the peak (29.27±0.90) at 8 hours after infection, and kept the high level for 24 and 48 hours (19.47±0.65) and (19.96±0.78), respectively. Conclusions iNOS is expressed persistently and effectively in hepatocytes during biliary tract infection. It is suggested that liver be the important organ synthesizing NO during biliary tract infection and have great influence over the outcome of infection.