胃癌组织ras和P53基因突变的检测

DETECTION OF MUTATION OF P53 GENE AND ras GENE IN STOMACH CANCER

1目的 研究胃癌组织中 P5 3基因和 ras基因的突变情况。 2方法 采用 PCR- RFL P技术 ,分析 41例胃癌标本中 P5 3基因和 ras基因的突变。 3结果 本组 41例中 ,12例 (2 9.3% )发生 P5 3基因突变 ,16例(39.1% )发生 ras基因突变 ,1例同时发生 P5 3基因和 ras基因的突变。4结论 P5 3和 ras基因突变与胃癌的发生密切相关 。

Objective\ To study the mutation of P53 gene and ras gene in stomach cancer.\ Methods\ The mutation of P53 gene and ras gene were analysed in 41 specimens of stomach cancer with PCR RFLP technology.\ Results\ The gene mutation was detected in 41 specimens (65.8%). Of 41 cases, the P53 gene mutation was 12(29.3%), the ras gene mutation was 16( 39.1% ), and the mutation of P53 gene and ras gene was only 1.\ Conclusion\ The gene mutation of P53 and ras is closely related to the occurrence of stomach cancer. [