泰乐菌素降解菌的分离鉴定及其系统发育分析

Isolation, Identification and Phylogenetic Analysis of a Tylosin-degrading Strain

发酵法生产泰乐菌素过程中产生的大量废弃药渣，因残留泰乐菌素的存在，会对环境带来不利影响．采用微生物法降解药渣中残留的泰乐菌素．结果表明：从堆放泰乐菌素药渣附近土壤中分离筛选到1株高效降解泰乐菌素的菌株，经16SrDNA鉴定为无丙二酸柠檬酸杆菌（Citrobacteramalonaticus）．该菌为好氧型革兰氏阴性杆菌，菌落直径0．5～1．5mm，可利用多种糖类、醇类和氨基酸作为碳源或氮源，具有较强的耐盐、耐酸、耐碱能力，对温度的适应范围较宽．用该菌株在30℃下处理含50mg／L泰乐菌素培养基72h，培养基中未检到泰乐菌素的存在．说明利用微生物法可有效降解药渣中残留的泰乐菌素．

A mass of pharmaceutical waste is produced in the process of tylosin production using microbial fermentation, and has a deleterious effect on the environment because of the existence of tylosin residue. The aim of this study was to investigate the degradation of tylosin residue in pharmaceutical waste by microbiological method. A tylosin-degrading strain was isolated from the soil deposited by tylosin pharmaceutical waste and identified as Citrobacter arnalonatieus based on 16S rDNA gene sequence. The isolate was aerobic gram-negative bacillus and the diameter of bacterial colony was 0.5-1.5 mm. This strain could make better use of the carbohydrates, alcohols and amino acids as carbon or nitrogen source and had an ability of resistance to salt, acid and alkali, and a wider adaptation to temperature. The tylosin in medium with an initial concentration of 50 mg/L was not detected after treatment with the isolated strain for 72 h under temperature 30 ℃. It suggested that tylosin residue in pharmaceutical waste could be degraded by using microorganism.