头花蓼枯萎病的病原鉴定及室内毒力测定

Pathogen Identification of Fusarium Wilt and Indoor Toxicity Determination of Polygonum capitatum

近年来贵州施秉县头花蓼发生一种枯萎病,该病害分布广、危害严重,已经成为影响当地头花蓼生产的潜在威胁。为了控制该病害的发生与危害,对头花蓼典型发病组织进行了病原菌分离鉴定和回接试验。结果表明,分离到的3种病原菌分别是弯孢属菌(Cochliobolus curvularia)、立枯丝核菌(Rhizoctoniasolani)和厚垣镰孢霉菌(Fusariumch lamydosporum)。经过接种试验,仅厚垣镰孢霉菌能引致与田间病害一致的症状,结合形态鉴定和ITS序列分析,初步确定该菌是头花蓼枯萎病的致病菌。室内抑菌试验表明,杀菌剂咪鲜胺、多菌灵、百菌清和恶霉灵对其有较好的抑制作用。

In recent years Fusarium wilt had a wide distribution and severe damage to P. capitatum in Shibing County in Guizhou, which had already been the potential threaten to local P. capitatum production. In this paper the pathogen in typical diseased tissue of P. capitatum was isolated and identified and a tie-back test was conducted to control the occurance and damage. The results showed that the three isolated pathogens were Cochliobolus lamydosporum. The inoculation test showed that curvularia , Rhizoctonia solani and Fusariumch only F. lamydosporurn could cause symptom in accordance with field disease. After morphological identification and ITS sequence analysis, it was preliminarily concluded that F. lamydosporum was the pathogenic bacterium. Bacteriostasis test showed that prochloraz, carbendazim, chlorothalonil and hymexazol had preferable inhibitory action on F. lamydosporum.