超声提取-液相色谱质谱法同时测定辣椒制品中5种非法添加染料

Determination of Five Illegally Used Dyes in Chili Products by Liquid Chromatography Mass Spectrometry with Ultrasonic Extraction

建立超声提取-液相色谱质谱法同时测定苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ以及罗丹明B五种食品非法添加染料的方法。辣椒制品以正己烷为提取溶剂,超声波提取15min,乙腈饱和正己烷溶液去油脂,乙腈定容。采用LC-MS/MS正离子扫描测定食品非法添加物罗丹明B和4种苏丹红。标准加标回收试验,罗丹明B添加浓度分别为2,4,8μg/kg,苏丹红Ⅰ～Ⅳ添加浓度为:10,20,30μg/kg,平均回收率为90.3%～94.3%,相对标准偏差(RSD)2.0%～4.9%。本方法5种非法添加染料的检出限罗丹明B为1μg/kg,苏丹红Ⅰ～Ⅳ检出限均为5μg/kg。该方法简便、快捷、大大降低了前处理时间,适合进行批量样品的测定。

A sensitive method was developed for the determination of Sudan red Ⅰ - Ⅳ and Rhodamine B in chili products by liquid chromatography mass spectrometry with ultrasonic extraction. N-hexane was used to extract the residues from chili products, and Acetonitrile saturated N-hexane was added into the extract to delete the fat. Sudan red Ⅰ - Ⅳ and Rhodamine B were determined using LC-MS/MS with the multiple reaction monitoring mode. Un- der optimized conditions, the average recoveries for sample spiked with the standards at 2,4,8 p,g/kg of Rhodamine B and the standards at 10, 20, 30 μg/kg of Sudan red Ⅰ - Ⅳ were 90.3 % - 94.3 % , the RSD was 2.0 % - 4. 9 %. The Rhodamine B limit of detection was 1μg/kg, and the Sudan red I - IV limit of detection was 5 μg/kg. Simple and convenient, the method shortened the time of pretreatment, and was fit for batched sample determination.