摘要
采用HPLC法测定罂粟茎、叶、壳部位中吗啡的含量,其色谱条件为:色谱柱HypersillC18(5μm,4.6nm×250nm);流动相:磷酸二氢钾(0.05mol/L):庚烷磺酸钠(0.0025mol/L):乙腈=(5:5:2);检测波长220nm。结果显示:罂粟植株中吗啡含量依次为:壳>叶>茎>;罂粟壳中吗啡的含量为2.567%-3.455%,叶中含量为1.742%-2.653%,茎杆中含量为0.128%-0.467%。
Determined the morphine content of poppy stems, leaves, shell parts by using the HPLC method, the chromatographic conditions were hypersillC18 （5 um, 4.6 nm.250 nm）; mobile phase ： potassium dihydrogen phosphate （ 0.05 mol/L ） ： sodium heptanesulfonate （ 0.0 025 mol/L ） ： acetonitrile = （5 ： 5 ： 2）; detecting wavelength 220 nm. The results showed that the poppy plant morphine content in turn were shell 〉 leaf 〉 stem 〉; the morphine content of poppy shell was 2.567%-3.455%, in leaves was 1.742%-2.653%, in stem was 0.128%-0.467%.
出处
《热带农业工程》
2012年第6期4-7,共4页
Tropical Agricultural Engineering
基金
甘肃省科技厅项目(No.098TTCH002)
(No.0708NKCH076)
关键词
野罂粟
吗啡
液相色谱法
wild poppy
morphine
liquid chromatography
