癫痫患儿不同时期脑脊液和血液中一氧化氮与氨基酸含量的变化

Level of Nitric Oxide and Amino Acids in Cerebrospinal Fluid and Serum of Epilepsy Children in Different Time

目的通过对癫痫患儿不同时间点脑脊液和血液中一氧化氮(NO)代谢产物NO2-与氨基酸水平的检测,阐明NO在小儿癫痫发病中的作用及临床意义。方法分别采用硝酸还原酶法、高效液相色谱(HPLC)法,检测癫痫发作后不同时间点脑脊液和血液中NO与氨基酸的含量。结果在惊厥发作后30 min、60 min、120 min,NO2-含量明显增高(P<0.01),而在惊厥发作后180min,NO2-含量渐降至正常(P>0.05)。在惊厥发作后30 min,谷氨酸(Glu)升高(P<0.05),而在惊厥发作后期,Glu的含量明显升高(P<0.01);甘氨酸(Gly)含量在癫痫发作各组逐渐下降(P<0.05)。结论 NO是内源性致痫物质,参与惊厥的点燃过程以及癫痫后期的氧化应激性脑损害过程。癫痫发作与中枢神经系统兴奋性氨基酸与抑制性氨基酸神经递质的平衡失调密切相关。兴奋性氨基酸的毒性作用是造成脑损害的主要原因之一。

Objective To detect the level of nitric oxide （NO） and amino acids in cerebrospinal fluid and serum of epilepsy children in different time. Methods The level of NO and amino acids in cerebrospinal fluid and serum of epilepsy children in different time were deter- mined with nitrate reductase and high performance liquid chromatography （HPLC） with fluorimetric detection. Results The level of NO2 in- creased 30 rain, 60 min, 120 min after seizures （P〈0.01）, but dropped to normal 180 rain after seizures （P〉0.05）. The level of glutamate （Glu） significantly increased in all epilepsy groups （P〈0.05）. The level of glycine （Gly） dropped gradually in all epilepsy groups （P〈0.05）. Conclusion NO may participate in the generation of seizures and the injury process to brain. The imbalance between excitatory and inhibito- ry amino acids may play an important role in the pathogenesis of epilepsy. The toxicity of excitatory amino acids may be the main injury to the brain.