生脉饮对TNF-α诱导的大鼠心肌成纤维细胞胶原合成的影响

Effect of Shengmai Oral Liquid on TNF-α-induced collagen synthesis in cardiac fibroblasts for rats

目的探讨生脉饮对肿瘤坏死因子(tumor necrosis factorα,TNF-α)诱导的新生大鼠心肌成纤维细胞(cardiacfibroblasts,CFs)胶原合成的影响及其机制。方法用不同浓度生脉饮载药血清与TNF-α处理体外培养的CFs,采用分光光度法检测细胞培养上清液中羟脯氨酸含量,采用实时定量RT-PCR检测MMP1和TIMP1 mRNA的表达水平。结果 TNF-α处理组羟脯氨酸含量较空白对照组明显升高;与TNF-α组比较,生脉饮口服液血清中羟脯氨酸含量降低。与空白对照组比较,TNF-α组MMP1和TIMP1 mRNA表达上调;生脉饮载药血清组MMP1较TNF-α组显著升高,而TIPM1显著降低,差异有统计学意义(均P<0.05),并呈浓度依赖性。结论生脉饮对TNF-α诱导的CFs胶原合成具有抑制作用,下调MMP1和上调TIPM1 mRNA表达可能是其作用的机制之一。

Objective To explore the effect and mechanism of Shengmai Oral Liquid on collagen synthesis of neonatal rat cardiac fibroblasts（CFs） induced by tumor necrosis factor（tumor necrosis factor α,TNF-α）.Methods Cardiac fibroblasts（CFs） were treated with different concentrations of Shengmai Oral Liquid drug-loaded serum plus TNF-α treatment in vitro.Hydroxyproline content,MMP1 mRNA and TIMP1 mRNA expression were assayed by spectrophotometry and real-time quantitative reverse transcription-PCR,respectively.Results The content of hydroxyproline in cardiac fibroblasts treated with TNF-α was higher than the control group.The content of hydroxyproline was reduced in Shengmai Oral Liquid drugloaded serum group compared with the TNF-α group.MMP1 and TIMP1 mRNA expression was up-regulated expression in TNF-α groups compared with the control group.MMP1 mRNA expression was up-regulated and TIMP1 was down-regulat ed in Shengmai Oral Liquid drug-loaded serum group compared with the TNF-α group.Conclusion The results reveal that Shengmai Oral Liquid can inhibit the collagen synthesis in cardiac fibroblasts,and may correlate with down-regulate expression of TIMP1 mRNA and up-regulate expression of MMP1 mRNA.