盐酸地芬尼多片含量的HPLC测定

Determination of Diphenidol Hydrochloride Tablets by HPLC

采用高效液相色谱法测定盐酸地芬尼多片的含量及含量均匀度。采用 YWG- C1 8色谱柱 ,以醋酸泼尼松为内标 ,甲醇 - 0 .5 %三乙胺溶液 (磷酸调 p H值为 3 .0 ) ( 62∶ 3 8)为流动相。方法灵敏度高 ,准确可靠 ,重现性好 ,平均回收率 99.94 % ,RSD为 0 .4 %。

The content and uniformity of dosage units of diphenidol hydrochloride in its tablets were determined by HPLC. Chromatographic conditions were as follows: column, YWG C 18 (4.6×250 mm); internal standard, prednisone acetate; mobile phase, methanol 0.5% triethylamine aqueous solution adjusted to pH 3.0 by H 3PO 4(62∶38); detection wavelength, 230 nm; flow rate, 1 ml/min. The average recovery was 99.94% with RSD of 0.4%.