高压氧对慢性疲劳综合征大鼠血管内皮功能的影响

Effect of hyperbaric oxygen on vascular endothelial function in rats with chronic fatigue syndrome

目的制作慢性疲劳综合征(chronic fatigue syndrome,CFS)大鼠模型,观察高压氧对CFS大鼠血管内皮功能,即血浆内皮素-1(endothelin-1,ET-1)、一氧化氮(nitric oxide,NO)及血管性假血友病因子(von wille-brand factor,vWF)的影响。方法将SD大鼠随机分为空白对照组、高压氧对照组、CFS模型组、CFS高压氧组及CFS休息组,采用负重力竭游泳的方法建立CFS动物模型,采用放射免疫法检测血清ET-1、NO及vWF水平。结果相对于空白对照组,CFS模型组血清ET-1、vWF升高(P<0.05),而NO降低(P<0.05);CFS高压氧组以上指标较CFS模型组呈反向变化,差异具有统计学意义(P<0.05)。结论慢性疲劳综合征大鼠血管内皮功能受损或功能紊乱,这种血管内皮功能障碍在高压氧治疗下能够得到积极改善。

Objective To produce chronic fatigue syndrome（chronic fatigue syndrome,CFS） rat model and to observe the effect of hyperbaric oxygen on CFS rats′vascular endothelial function,as indicated by plasma endothelin-1（ET-1）,nitric oxide（NO） and von wille-brand Factor（vWF）.Methods SD rats were randomly assigned to blank control group,hyperbaric oxygen control group,the CFS model group,the CFS hyperbaric oxygen group and the CFS rest group.CFS animal model was established by the load method of exhaustive swimming and ET-1,NO and vWF levels were measured by the radioimmunoassay of serum.Results Serum ET-1 and vWF levels were higher in the CFS model group than those of the control group（P 0.05）,while NO level was lower（P 0.05）;the indicators showed a reverse change in the CFS hyperbaric oxygen group compared with the CFS model group（P 0.05）.Conclusion Rats with chronic fatigue syndrome show endothelial dysfunction,which can be improved by hyperbaric oxygen therapy in model animal.