多胺化壳聚糖微球固定化AS1.398中性蛋白酶

Immobilization of AS1.398 Neutral Proteinase on polyamine Modified Chitosan Microspheres

从自制的单分散微米级壳聚糖微球出发,经C2氨基苯甲醛保护、C6羟基环氧化后接枝亲水性多胺,进一步制备多胺化壳聚糖载体,采用该载体对AS1.398中性蛋白酶进行固定化.结果表明:制备的固定化酶活力回收率达49%以上,是采用未改性壳聚糖微球固定化的2.7倍.固定化酶连续重复使用9批次后仍保留有原活力的一半.

Monodisperse chitosan microspheres in the range 60 μm to 200 μm were prepared as support matrix for immobilized enzyme by phase-inversion suspension cross-linking technique firstly,then polyethylenamine modified chitosan microspheres with more amino group content and better mechanical resistance were prepared.AS1.398 neutral proteinase was immobilized on the support.The recovery of immobilized AS1.398 neutral proteinase activity reached 49 %,which was 2.7 times that as immobilization on pure chitosan microsphere.