孕早期人母-胎免疫耐受机制的研究

Investigation of materno fetal immunotolerance in human first trimester pregnancy

目的 :探讨孕早期母胎免疫耐受调节机制。方法 :应用流式细胞仪分析 (FACS)蜕膜及孕妇外周血淋巴细胞亚群 ;采用改进的乳酸脱氢酶释放试验测定蜕膜淋巴细胞对K5 6 2 的自然杀伤活性 ;同时以滋养细胞与蜕膜淋巴细胞共同培养 ,研究母体免疫系统对滋养细胞抗原免疫应答状态。结果 :蜕膜组织中CD5 6 + NK细胞含量高于孕妇及对照组外周血CD5 6 + 细胞比例 (P <0 .0 5 ) ;蜕膜组织中NK细胞杀伤活性明显低于孕妇及对照组外周血NK细胞杀伤活性 (P <0 .0 5 ) ;滋养细胞抑制母方淋巴细胞增殖反应 (P <0 .0 5 )。结论 :滋养细胞调低蜕膜淋巴细胞增殖反应 ,蜕膜CD5 6 + NK细胞自然杀伤活性受抑制 。

Objective:To investigate materno fetal immunoregulation mechanism in human first trimester pregnancy.Methods:The subsets of decidual lymphocytes and peripheral blood lymphocytes(PBL) were analyzed by FACS test. The proliferative reaction of a mixed culture of decidual lymphocytes to trophocytes was investigated in an assay analogous to one way mixed lymphocytes reaction.Results:CD56 + cells of first trimester decidual were higher than those of PBL from the pregnant women and the healthy fertile women(P<0.05). The NK cytotoxicity of first trimester decidual is lower than those of PBL from pregnant woman and control woman (P<0.05). Trophocytes down regulated the proliferation of decidual lymphocytes (P<0.05).Conclusion:It is due to the interaction between trophocytes and CD56 + NK cells in decidual that the materno fetal immunotolerance is maintained.