去甲斑蝥素诱导K562细胞凋亡的影响

ABSTRACTS OF ORIGINAL ARTICLES

目的:探讨去甲斑蝥素(NCTD)对人白血病细胞株K562细胞增殖的抑制作用。方法:用流式细胞仪测定NCTD对K562细胞体外培养过程中细胞毒性、细胞凋亡及细胞周期的影响。结果:NCTD对K562细胞有较强的增殖抑制作用;在作用24小时后达到凋亡高峰;24小时各浓度NCTD作用后,G_1期细胞百分数均减少,S期与G_2+M期细胞百分数均增加,呈现G_2+M期阻滞现象。结论:NCTD可诱导K562细胞凋亡,抑制瘤细胞分裂增殖,且有明显的时间和剂量效应。

To study the inhibitory effect of norcantharidin (NCTD) on the proliferation of K562 cells. The effect of NCTD on the cell toxicity, cell cycle and cell apoptosis of K562 cells were detected by flowcytometry when cultured in vitro. NCTD has inhibiting effect on the proliferation of K562 cells, and could induce the cells apoptosis which reached to the high level after 72 hour. As 10μM NCTD, G1 phase arrest was revealed by the increase of cells in G1 phase and decrease in both the S phase and G2 + M phase. NCTD can induce the apoptosis of K562 cells, and inhibit the proliferation of K562 cells, the effects appearing markedly correlated with time and dosage.