摘要
目的建立肝中杀鼠酮的硅藻土提取方法和高效液相色谱检测法。方法取0.5g肝匀浆,加6%HClO4沉淀蛋白,准确取1/2上清液倒入装有3.1g硅藻土的层析柱中,用10mL二氯甲烷或乙醚洗脱,洗脱液中加入安定作为内标,水浴浓缩,用0.2mL甲醇定容,供高效液相色谱分析。结果提取率分别为98.1%和99.9%,检出限分别为14ng/mL和13ng/mL。结论该方法简便、快速、提取率高,可作为法医毒物常规检测手段。
Objective To establish a method for determining Valone in liver. Methods Valone in liver was extracted in diatomite column with codichloromethane and ether respectively, and then the extraction was analyzed by high performance liquid chromatography. Results The extraction rate were 98. 1% and 99. 9%, the limits of detection were 14ng/mL and 13ng/mL. Conclusion The method is simple, rapid, high extraction rate and can be used as a conventional method of forensic toxicology.
出处
《刑事技术》
2012年第6期45-46,共2页
Forensic Science and Technology
关键词
杀鼠酮
肝
高效液相色谱法
硅藻土
Valone high performance liquid chromatography
diatomite
