偏心旋转刺激下大鼠前庭核c-fos表达的观察

c-fos Expression in Rat’s Vestibular Nucleus Under Eccentric Rotational Stimulation.

观察偏心旋转刺激下前庭核早期即刻基因c fos的表达特征和抗运动病药物对这种表达的影响。方法取SD大鼠 1 9只分为对照组、旋转组、药物组三组。旋转组用偏心旋转刺激方式刺激60min ;药物组在偏心旋转刺激前 45min腹腔注射抗运动病药物盐酸扁桃桥哌酯 (PAPM ) ;对照组不进行旋转刺激。用免疫组化法和图象分析技术对大鼠脑干前庭神经fos蛋白含量变化进行定量、定位测定。结果偏心旋转刺激后前庭核团细胞核中fos蛋白含量明显增加 ,而PAPM并不影响fos蛋白的表达。结论前庭核的c fos表达是前庭神经系统对外界刺激应答的一种方式 ;且这种表达不直接参与运动病的发生和发展。

Objective To investigate the c fos expression in rat’s vestibular nucleus under eccentric rotational stimulation and the effects of anti motion sickness drug(PAPM) on this expression. Method 19 SD rats were divided into three groups: A,B and C.A received as control. B was stimulated by eccentric rotation for 60 minutes. C received injection of PAPM through cavun abdominis 45 min before eccentric rotation. Immunohistochemical method and computerized image analysis were used to map locations of c fos protein appeared in four vestibular nucleus and to count the masculine cells. Result c fos protein appeared in four vestibular nucleus areas after stimulation, and PAPM had no influence on this expression. Conclusion It suggests that c fos expression in vestibular nucleus is one of the important way in which vestibular nervous system reacts to outside stimulation and this expression has no direct relationship with the generation and development of motion sickness.