碱性蛋白酶酶解高温豆粕技术的研究

Research on technology of enzymolysis high temperature bean pulp with alkaline proteinase

以高温豆粕为原料,通过单因素试验、正交试验进行直观分析和方差分析,确定碱性蛋白酶酶解高温豆粕的优化条件。以大豆肽水解度为指标,考察温度、时间、pH、加酶量、底物浓度等因素对水解度的影响。优化的酶解条件为:温度55℃、酶解时间为4h、pH为9.0、加酶量为9 600U/g(底物)、底物浓度为10%,在此条件下做验证试验得到的大豆肽水解度为34.93%。

Taking high temperature bean pulp as raw material, single factor test, orthogonal. to the visual analysis and variance analysis to determine the alkaline protease enzyme optimal tem- perature conditions for soybean meal. Degree of hydrolysis of soybean peptide as an indicator of temperature, time, PH, enzyme amount, substrate concentration on the impact of the degree of hydrolysis. Optimization of hydrolysis conditions.＂ temperature： 55 ℃, time： 4 h, pH： 9.0, en- zyme dosage： 9 600 U. g^-1 （substrate）, substrate concentration： 10%. In this condition, the high temperature hydrolysis to confirm the test meal obtained soybean peptide hydrolysis was 34.93 %.