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缺血预处理对脑缺血耐受大鼠血管内皮生长因子和血管生成素-1表达的影响 被引量：1

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摘要 目的探讨短暂性脑缺血预处理(IP)对脑缺血耐受大鼠血管内皮生长因子(VEGF)和血管生成素-1(Ang-1)蛋白表达的影响。方法将99只Wistar大鼠随机分成IP组(45只)、非缺血预处理(NIP)组(45只)和假手术对照组(9只)。首先应用线栓法阻塞大鼠大脑中动脉,建立局灶性缺血预处理模型,然后分别在缺血预处理后第1,3,7,14,21天给予再次缺血2 h再灌注22 h处理,根据缺血-再灌注时间分别将NIP组及IP组再分成5个亚组,NIP组以假手术代替预缺血,假手术对照组两次均为假手术。采用TTC染色测定脑梗死体积,免疫组织化学法检测缺血侧皮层和纹状体VEGF和Ang-1蛋白的表达。结果 IP组大鼠第1,3,7d亚组脑梗死体积明显小于NIP组(均P<0.01),其中以IP组第3天亚组脑梗死体积减小最为明显。IP组第1,3,7天亚组VEGF蛋白表达水平显著高于NIP组(均P<0.05),其中以IP组第3天亚组最为明显。假手术对照组和NIP组均存在一定程度Ang-1蛋白表达,但IP组第7天亚组Ang-1蛋白表达明显增高(P<0.05)。结论预缺血诱导VEGF及Ang-1蛋白表达增加,对脑缺血耐受的产生发挥重要作用。
作者 丛丽 王春霞 赵仁亮
机构地区 青岛市市立医院麻醉科 青岛市精神卫生中心 青岛大学医学院附属医院神经内科
出处 《中国医药指南》 2012年第35期449-451,共3页 Guide of China Medicine
关键词 缺血耐受 血管内皮生长因子 血管生成素-1 缺血预处理
分类号 R-33 [医药卫生]
  • 相关文献

参考文献10

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共引文献3

同被引文献13

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中国医药指南
2012年 第35期

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