期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

hTERT启动子引导hNIS基因重组腺病毒介导放射性核素的裸鼠显像 被引量：2

Transfected sodium iodide symporter gene following human telomerase reverse transcriptase promoter for tumor imaging of malignant glioma nude mice
下载PDF
导出
摘要 目的:在裸鼠体内实验中,观察使用人端粒酶逆转录酶(hTERT)核心启动子引导人钠碘转运体(hNIS)基因的重组腺病毒介导放射性核素显像的可行性。方法:扩增重组腺病毒,测定转染后U87细胞摄碘率,构建U87人胶质瘤荷瘤裸鼠模型,使用重组腺病毒Ad-hTERT-hNIS进行体内转染,进行放射性核素99Tcm显像。结果:重组腺病毒Ad-hTERT-hNIS转染端粒酶阳性的U87细胞后,hNIS基因能够介导胶质瘤细胞摄碘,并且该病毒能对U87胶质瘤荷瘤裸鼠进行体内转染;成功介导靶向性hNIS基因的表达,而转染后的荷瘤裸鼠模型能成功介导放射性核素99Tcm04-显像。结论:重组腺病毒Ad-hTERT-hNIS能够实现裸鼠体内hNIS基因的靶向性表达,并能介导放射性核素99Tcm显像。 Objective： To investigate the possibility of transfecting human sodium iodide symporter （hNIS） gene following human telom- erase reverse transcriptase promoter （hTPO） into U87 malignant glioma nude mice for radioactive nuclide imaging in vivo. Methods： The recombinant adenovirus were amplified and purified, and the ^125I-iodide uptake of transfected U87 cells was detected. The malignant glioma nude mice animal model was performed, Ad-hTERT-hNIS was transfected in vivo, then radioactive nuclide ^99Tcm tumor imaging was obtained. Results： After U87 cells of telomerase-positive were transfected with Ad-hTERT-hNIS, Ad-hTERT-hNIS could mediate ^125I uptake of malignant glioma cell and malignant glioma nude mice animal model could be transfected by Ad-hTERT-hNIS, result in tumor specific hNIS gene expressioning and radioactive nuclide ^99Tcm tumor imaging. Conclusion： The recombinant adenovirus Ad- hTERT-hNIS could lead tumor specific hNIS gene expression in malignant glioma nude mice animal model and show ^99Tcm tumor imaging.
作者 赵兴业 李玮 谭建 王澎 李宁
机构地区 天津医科大学总医院核医学科
出处 《天津医科大学学报》 2012年第4期422-424,共3页 Journal of Tianjin Medical University
基金 国家自然科学基金资助项目(81171372)
关键词 NIS基因 胶质瘤 重组腺病毒 放射性核素 裸鼠 sodium iodide symporter malignant glioma recombinant adenovirus radioactive nuclide nude mice
分类号 Q7 [生物学—分子生物学] R445 [医药卫生—影像医学与核医学]
  • 相关文献

参考文献15

  • 1Kang J H, Chung J K. Molecu|ar-genetic imaging based on reporter gene expression[J]. J Nucl Med, 2008, 49(Suppl 2) :S164.
  • 2Harney A S, Meade T J. Molecular imaging of in vivo gene expres- sion[J]. Future Med Chem, 2010, 2(3 ):503.
  • 3郑薇,谭建,李玮,李宁,许静.裸鼠模型的人钠/碘转运体基因转染人大细胞肺癌介导放射性核素显像[J].中国医学影像技术,2009,25(1):7-9. 被引量：3
  • 4Omay S, Vogelbaum M. Current concepts and newer developments in the treatment of malignant gliomas[J]. Indian J Cancer, 2009, 46 (2):88.
  • 5Lim S K, Llaguno S R, McKay R M, eta|. Glioblastoma muhiforme: a perspective on recent findings in human cancer and mouse models [J]. BMB Rep, 2011, 44(3):158.
  • 6Kim K, Kang J, Chung J, et al. Doxorubicin enhances the expression of transgene under control of the CMV promoter in anaplastic thy- roid carcinoma cells[J]. J Nuel Med, 2007, 48(9 ) :1553.
  • 7Ma X, Huang R, Kuang A. AFP promoter enhancer increased spe- cific expression of the human sodium iodide symporter (hNIS) for targeted radioiodine therapy of hepatocellular carcinoma[J]. Cancer Invest. 2009. 27 ( 6 ):673.
  • 8裴之俊,张永学.报告基因显像监测干细胞治疗的研究进展[J].国际放射学核医学杂志,2010,34(2):73.
  • 9Liu H, Ren G, Liu S, et al.Optical imaging of reporter gene expres- sion using a positron-emission-tomography probe[J]. J Biomed Opt, 2010, 15 ( 6 ) :060505.
  • 10Zuckier L S, Dohan O, Li Y, et al. Kinetics of perrhenate uptake and comparative biodistribution of perrhenate, pertechnetate, and iodide by NaI symporter-expressing tissues in vivo[J]. J Nucl Med, 2004, 45 ( 3 ):500.

二级参考文献13

  • 1Petrich T, Knapp WH, Potter E. Functional activity of human sodium/iodide symporter in tumor cell lines. Nuklearmedizin, 2003 42(1) :15 -18.
  • 2Cho JY, Shen DHY, Yang W, et al. In vivo imaging and radioiodine therapy following sodium iodide symporter gene transfer in animal model of intracerebral gliomas. Gene Ther, 2002, 9 (17): 1139-1145.
  • 3Smit JW, Schroder-van der Elst JP, Karperien M, et al. Expression of the human sodium/iodide symporter (hNIS) in xenotrans-plated human thyroid carcinoma. Exp Clin Endocrinol Diabetes, 2001,109(1) :52 55.
  • 4Dadachova E, Carrasco N. The Na/I symporter (NIS) : imaging and therapeutic applications. Semin Nucl Med, 2004, 34 ( 1 ) : 23- 31.
  • 5Kosugi S, Okamoto H, Tamada A, et al. A novel peculiar mutation in the sodium/iodide symporter gene in Spanish siblings with iodide transport defect. J Clin Endocrinol Metab, 2002, 87(8):3830-3836.
  • 6Wapnir IL, van de Rijn M, Nowels K, et al. lmmunohistochemical profile of the sodium/iodide symporter in thyroid, breast, and oth er carcinomas using high density tissue microarrays and eonven tional sections. J Clin Endocrinol Metab, 2003,88(4) : 1880-1888.
  • 7Zhang L, Sharma S, Zhu LX, et al. Nonradioactive iodide effectively induces apoptosis in genetically modified lung cancer cells. Cancer Research, 2003(63) :5065-5072.
  • 8Spitaweg C, Morris JC. Gene therapy for thyroid cancer: current status and future prospects. Thyroid, 2004,14(6) :424-434.
  • 9Cengic N, Baker CH, Sehutz M, et al. A novel therapeutic strate gy for medullary thyroid cancer based on radioiodine therapy following tissue specific sodium iodide symporter gene expression. J Clin Endocrinol Metab, 2005,90(8):4457 -4464.
  • 10Faivre J, Clerc J, Gerolami R, et al. Long term radioiodine retention and regression of liver cancer after sodium iodide symporter gene transfer in wistar rats. Cancer Res, 2004,64(21) : 8045- 8051.

共引文献2

同被引文献14

  • 1Dai G, Levy O, Carrasco N. Cloning and characterization of the thy- roid iodide transporter[ J ]. Nature, 1996,379 (6564) :458 - 460.
  • 2Joseph C Wu. Molecular imaging antidote to cardiac stem cell contro- versy[J]. JAm CoIl Cardiol,2008,52(20) :1661-1664.
  • 3Terrovitis J, Kwok KF, Lautamtiki R, et al. Ectopic expression of the sodium - iodide symporter enables imaging of transplanted cardiac stem cells in vivo by single - photon emission computed tomography or positron emission tomography [ J ]. J Am Coil Cardiol,2008,52 (20) : 1652 - 1660.
  • 4Lee KH, Bae JS, Lee SC,et al. Evidence that myocardial Na/I sym- porter gene imaging does not perturb cardiac function [ J]. J Nucl Med,2006,47(11) :1851 - 1857.
  • 5Schirmer JM, Miyagi N, Rao VP,et al. Recombinant adeno - associat- ed virus vector for gene transfer to the transplanted rat heart [ J ]. Transpl Int,2007,20 ( 6 ) :550 - 557.
  • 6Spitzweg C, O'Connor MK, Bergen ER, et al. Treatment of prostate cancer by radioiodine therapy afier tissue - specific expression of the sodium iodide symporter[ J]. Cancer Res, 2000,60 (22): 6526 - 6530.
  • 7Dwyer RM, Berger ER, O'Connor MK, et al. In vivo radioiodide im- aging and treatment of breast cancer xenografts after MUC1 - driven expression of the sodium iodide symporter[ J ]. Clin Cancer Res, 2005,11 (4) : 1483 - 1489.
  • 8Dingli D, Diaz RM, Bergert ER, et al. Genetically targeted radiother- apy for multiple myeloma[ J ]. Blood, 2003,102 (2) :489 - 496.
  • 9Scholz IV, Cengic N, Baker CH, et al. Radioiodine therapy of colon cancer following tissue - specific sodium iodide symporter gene transfer [ J ]. Gene Ther,2005,12 ( 3 ) :272 - 280.
  • 10Cengic N, Baker CH, Schutz M, et al. A novel therapeutic strategy for medullary thyroid cancer based on radioiodine therapy following tis- sue - specific sodium iodide symporter gene expression [ J]. J Clin Endocrinol Metab, 2005,90( 8 ) :4457 - 4464.

引证文献2

天津医科大学学报
2012年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部