Src酪氨酸激酶抑制剂对人胃癌细胞SGC7901上皮-间质转变相关基因的作用及机制

Inhibition effect and mechanism of Src tyrosine kinase inhibitor on EMT in human stomach cancer cell line SGC7901

目的:探讨Src酪氨酸激酶抑制剂对人胃癌细胞SGC7901上皮-间质转变相关基因的作用及机制。方法:以Src酪氨酸激酶抑制剂作用前后的SGC7901细胞为研究对象,应用Western blot及real time PCR观察细胞内p-Src及上皮间质转变标志物E-钙黏素(E-cadherin)、β-连环蛋白(β-catenin)表达的变化,报告基因技术检测Snail在转录水平表达的变化。结果:Src酪氨酸激酶抑制剂可上调E-cadherin在mRNA和蛋白水平变化,下调β-catenin在mRNA和蛋白水平变化及Snail的启动子活性。结论:Src酪氨酸激酶抑制剂可抑制人胃癌细胞SGC7901上皮间质转变相关基因表达,其机制可能与其下调SGC7901细胞Snail的启动子活性有关。

Objective： To evaluate the inhibiting effect and mechanism of Src tyrosine kinase inhibitor on EMT in human stomach cancer SGC7901 cell line. Methods： 4-anilinoquirazoline was used to inhibit Src tyrosine kinase in human stomach cancer SGC7901 cell line, western blot assay and real-time PCR were used to examine the expression of p-Src, E-cadherin and β-catenin at mRNA and protein level, reporter genes assay was used to examine the transcriptional activity of Snail. Results： Src tyrosine kinase inhibitor 4-anilinoquira- zoline at 5 μmol/L and 10 μmol/L obviously increased the expression of E-cadherin at protein and mRNA levels （P〈0.05）, and decreased the expression of β-catenin at protein and mRNA levels （P〈0.05）, transcriptional activity of Snail （P〈0.05）. Conclusion： Src tyrosine ki- nase activity is related with the EMT potential of SGC7901 cell line, the possible mechanism of the EMT inhibition effect of Src tyrosine kinase inhibitor may be the change of Snail transcriptional activity.