儿童过敏性紫癜补体活性研究

Research of complement activity in children with anaphylactoid purpura

目的探讨过敏性紫癜患儿在急性期和恢复期血清补体C3、C4活性。方法选取2009年10月～2011年11月我院接诊治疗的过敏性紫癜患儿97例为研究对象．按其临床表现分为皮肤型组、腹型组、肾型组、关节型组和混合型组；按照其病情进展分为急性期和恢复期，分别测定患儿的血清补体C3、C4水平。同时，与32例健康儿童组成的对照组同期测定的补体水平做对比分析。检测方法为应用美国Beckman—coulter公司的Array360型散射免疫比浊仪，采用免疫散射速率比浊法进行检测。结果过敏性紫癜患儿在急性期和恢复期血清补体C3、C4水平在不同临床分型组问差异无统计学意义（P〉0．05）；急性期、恢复期与对照组相比，血清补体C4水平差异无统计学意义（P〉0．05）；与对照组相比，急性期皿清补体C3水平降低（P〈0．05），恢复期C3水平相对急性期升高，但仍较对照组低（P〈0．05）。结论不同临床分型过敏性紫癜患儿其血清补体水平无明显差异；而相对于健康患儿其急性期补体C3水平降低，恢复期相对同升，而血清补体C4水平无变化。

Objective To investigate the complement C3, C4 activity in children with anaphylactoid purpura of acute and convalescent phase. Methods 97 children with anaphylactoid purpura treated in our hospital from October 2009 to November 2011 were selected as research objects. According to the clinical manifestation, all patients were divided into the skin-type group, abdominal-type group, kidney-type group, joint-type group and mixed-type group. According to the progression disease, patients were divided into the acute phase and the convalescent phase. At the same time, the complement levels were compared with that 32 cases of healthy children, who were taken as the control group. Then the children serum C3, C4 level were detected by immune scattering rate turbidimetric method using Array 360 scattering immune turbidimetrie instrument produced in Beckman-coulter company. Results In the acute and convalescent phase of anaphylaetoid purpura children, the serum C3 and C4 levels among different clinical type groups had no difference （P 〉 0.05）. The serum C4 level in acute, convalescent phase groups and control group had no statistically significant difference （P 〉 0.05）; but compared with the control group, the C3 level in acute phase reduced （P 〈 0.05）, which increased in convalescent phase than that in acute phase, but still lower than that of the control group （P 〈 0.05）. Conclusion The serum complement level in anaphylactoid purpura children with different clinical types have. no obvious difference. But compared with heahhy children, the C3 level in acute phase reduced, it relatively picks up in convalescent phase, then the level of serum complement C4 has no change.