动脉内注入蒸馏水并高脂喂养后大鼠的动脉内膜变化

The arterial intimal changes of rats with intra-arterial injection of distilled water and fed with high-fat diets

目的:观察动脉内注入蒸馏水并高脂喂养后大鼠动脉内膜的变化,以探讨动脉内膜损伤动物模型的建立方法以及进一步了解动脉硬化闭塞的发病机制。方法:在大鼠左后肢股-腘动脉内注入蒸馏水造成动脉内膜非机械性、浅表性损伤,再以高脂饲料喂养;于内膜损伤术后7,15,30,90 d时,取左后肢股-腘动脉,行HE等染色,光镜下观察。结果:第7天见局部内皮细胞大量脱落;第15天见内皮细胞完全脱落;第30天见内皮增厚,管腔狭窄;第90天见内膜增厚、部分钙化和血栓形成、管腔闭塞。结论:大鼠股-腘动脉内注入蒸馏水再以高脂饲料喂养较短时间内可形成动脉硬化闭塞。

Objective： To assess the arterial intimal changes of rats, which were injured with intra-arterial injection of distilled water and fed with high-fat diets, for a further understanding of the pathogenesis of arteriosclerosis obliterans. Methods： After distilled water was injected into the left side of femoropopliteal artery form the non- mechanical, superficial injury, rats were fed with high-fat diets. At 7, 15, 30 and 90 d after the intimal injury, the femoropopliteal artery of the left hind limb was excised. HE and other staining were performed, and the results were observed by light microscope. Results： A large number of endothelial cells exfoliated after 7 d; endothelial cells completely exfoliated after 15 d. Endothelial thickening and lumen stenosis was observed after 30 d and endothelial thickening, partial calcification, thrombus formation and luminal occlusion were noted after 90 d. Conclusion： Injection of distilled water into the femoropopliteal artery of rats, and then fed with high-fat diets can form an arteriosclerosis obliterans model in a relatively short period of time.