滇南小耳猪生长激素基因多态性研究

Studies on the Polymorphism of Growth Hormone Gene in Diannan Small-ear Pig

目的探讨滇南小耳猪生长激素基因多态性,为滇南小耳猪的选育提供分子标记辅助选择的依据.方法采用PCR-RFLP方法对滇南小耳猪GH基因的ApaⅠ酶切位点多态性进行了分析.结果检测到两种基因型AA和AB,AB型的频率高子AA型.没有检测到BB基因型个体.等位基因A的基因频率高于等位基因B,等位基因A在群体中占优势.结论滇南小耳猪群生长激素基因具有较丰富的多态性.

Objective To provide foundation for molecular marker-assisted selection in Diannan small-ear pig through investigating the gene polymorphism of growth hormone.Method The polymorphism of pGH gene was investigated by using PCR-RFLP at ApaⅠrestriction loci.Results Genotypes AA and AB were detected.The freyuency of genotype AB was higher than AA.Genotype BB was not detected.The allele A frequency was higher than B,A was dominant in Diannan small-ear pig colony.Conclusion The growth hormone has gene polymorphism in Diannan small-ear pig.