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RNA干扰的途径和机制 被引量：1

Pathway and Mechanism of RNA Interference
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摘要 RNA干扰(RNA interference,RNA)i是由双链RNA介导的序列特异的基因沉默现象,它在转录水平、转录后水平和翻译水平上阻断基因的表达,具有高效性和高特异性的特点.现就RNAi的两种途径:小干扰RNA和微小RNA的特点和机制作一综述. RNA interference（RNAi）is sequence-specific gene silencing phenomena mediated by double-stranded RNA,and it can block gene expression at the level of transcription,post-transcription and translation,with high efficiency and high specificity.The characteristics and mechanisms of the two pathways of RNAi,including short interfering RNA and microRNA,are reviewed in this paper.
作者 李绍祥 郭汉斌 曹建彪
机构地区 北京军区总医院全军肝病治疗中心
出处 《昆明医科大学学报》 CAS 2012年第11期148-151,共4页 Journal of Kunming Medical University
基金 国家自然科学基金资助项目(30872353)
关键词 RNA干扰 小干扰RNA 微小RNA RNA interference Short interfering RNA MicroRNA
分类号 R392.13 [医药卫生—免疫学]
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参考文献23

  • 1KANELLOPOULOU C,MULJO S A,KUNG A L,et al. D- icer-deficient mouse embryonic stem ceils are defective in differentiation and centromeric silencing [ J ]. Genes Dev, 2005,19(4) :489 - 501.
  • 2LEE Y,KIM M, HAN J,et al. MicroRNA genes are trans- cribed by RNA polymerase II [J]. EMBO J, 2004 , 23 :4 051 - 4 060.
  • 3LUND E,GITTINGER S, CALADO A,et al. Nuclear exp- ort of microRNA precursors [J]. Science , 2004 , 303 (5 654):95--98.
  • 4BARTEL D P. MicroRNAs genomics, biogenesis, mecha - nism, and function [ J ]. Cell, 2004, A (2): 281 -- 297.
  • 5JOPLING C L, YI M, LANCASTER A M, et al. Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA[ J~.Science, 2005,309(5 740) : 1 577-- 1 581.
  • 6HAMPTON T. MicroRNAs linked to pancreatic cancer[ J ]. JAMA,2007,297 (9) :9 371.
  • 7潘秋卫,蔡荣,刘新垣,钱程.microRNA与肿瘤发生、诊断及治疗[J].自然杂志,2006,28(2):84-87. 被引量：6
  • 8ZHANG L,COUKOS G. MicroRNAs: a new insight intocancer genome[J].Cell Cycle, 2006,5(19) :22 162-- 22 191.
  • 9SAMOLS M A, HU J, SKALSKY R L,et al. Cloning and identifieation of a microRNA cluster within the lateney-associated region of Kaposi' s sarcoma-assoeiated herpesvirus[J]. J Virol,2005,79(14) :9 301 --9 305.
  • 10SULLIVAN C S, GRUNDHOFF A T,TEVETHIA S, et al. SV40-encoded microRNAs regulate viral gene expression and reduce susceptibility to cytotoxic T cells [J]. Nature, 2005,435(7 042) :682--686.

二级参考文献21

  • 1KRUTZFELDT J,RAJEWSKY N,BRAICH R,et al.Silencing of microRNAs in vivo with 'antagomirs'[J].Nature,2005,438(7068):685-689.
  • 2WEILER J,HUNZIKER J,HALL J.Anti miRNA oligonucleotides (AMOs):ammunition to target miRNAs implicated in human disease[J] ? Gene Ther,2005 Sep 29[Epub ahead of print].
  • 3LIU X Y,GU J F,SHI W F.Targeting gene-virotherapy for cancer[J].Acta Biochim Biophys Sin (Shanghai),2005,37(9):581-587.
  • 4PEI Z,CHUN L,ZOU W,et al.An oncolytic adenoviral vector of Smac increases antitumor activity of TRAIL against HCC in human cells and in mice[J].Hepatology,2004,39(5):1371-1381.
  • 5ZOU W,LUO C,ZHANG Z.Novel oncolytic adenovirus targeting to telomerase activity in tumor cells with potent[J].Oncogene,2004,23(2):457-464.
  • 6SILVA J M,LI M Z,CHANG K,et al.Second generation shRNA libraries covering the mouse and human genomes[J].Nat Genet,2005,37(11):1281-1288.
  • 7STEGMEIER F,HU G,RICKLES R J,et al.A lentiviral microRNA-based system for single-copy polymerase II regulated RNA interference in mammalian cells[J].Proc Natl Acad Sci USA,2005,102(37):13212-13217.
  • 8GREGORY R I,SHIEKHATTAR R.MicroRNA biogenesis and cancer[J].Cancer Res,2005,65(9):3509-3512.
  • 9CALIN G A,DUMITRU C D,SHIMIZU M,et al.Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia[J].Proc Natl Acad Sci U S A,2002,99(24):15524-15529.
  • 10JOHNSON S M,GROSSHANS H,SHINGARA J,et al.RAS is regulated by the let-7 microRNA family[J].Cell,2005,120:635-647.

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昆明医科大学学报
2012年 第11期

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