山羊乳牙牙髓干细胞在肌袋内的异位成骨

Ectopic osteogenesis of SGDs in goat's muscle pouch

目的:评价山羊乳牙牙髓干细胞复合多孔磷酸钙骨水泥在山羊肌袋模型内的异位成骨能力。方法:采用改良组织块法培养山羊乳牙牙髓干细胞。经成骨诱导条件培养的第3代细胞与多孔磷酸钙骨水泥复合后,植入山羊背部左侧肌袋,将多孔磷酸钙骨水泥空白支架植入右侧肌袋作为对照组。分别在植入后2、4、6、8周取材,进行组织学观察,采用SPSS16.0软件包对数据进行统计学分析。结果:术后各时间点未复合细胞的pCPC支架材料组织学检查未见类骨质形成。SGDs-pCPC组中,术后2、4、6、8周成骨率分别为(1.24±0.25)%、(1.59±0.23)%、(4.12±0.39)%和(5.68±0.58)%。术后2周和4周之间的成骨率无显著差异(P>0.05);术后8周的成骨率高于术后6周,且2者均显著高于2周和4周组的成骨率(P<0.05)。结论:多孔磷酸钙复合山羊乳牙牙髓干细胞在山羊体内具有异位成骨能力。

PURPOSE： To investigate ectopic osteogenesis of composites SGDs with porous calcium phosphate cement （pCPC） in goat＇s muscle pouch. METHODS： SGDs were cultivated with modified tissue culture techniques, then were induced into osteoblasts in the third passage, the osteogenic-induced SGDs were combined with pCPC and transplanted into the goat left dorsal muscle pouch, the pCPC without cells was transplanted into the right dorsal muscle pouch as negative controls. The transplants were harvested at 2-,4-,6-,8- week and prepared for histological examination. The morphologic quantitative analysis was made by SPSS 16.0 software package. RESULTS： Bone formation was not detected in pCPC without cells by histological examination. 2,4,6,8 weeks after transplantation in SGDs-pCPC group, the percentages of bone formation were （ 1.24＋0.25 ）%, （ 1.59＋0.23 ）%, （4.12_＋0.39）% and （5.68_＋0.58） %,respectively.There was no significant difference in bone formation at 2 and 4 weeks after transplantation （P〉0.05）. At 8- week, the percentages of bone formation were higher than that at 6- week in SGDs-pCPC group, and both significantly higher than that at 2- and 4- week （P〈0.05）. CONCLUSION： SGDs combined with pCPC have the ability of ectopic osteogenesis. Supported by Research Fund of Science and Technology Commission of Shanghai Municipality （09JC1411700）, Research Foundation of Shanghai Jiao Tong University School of Medicine （09XJ21030） and Collaborative Projects of Development and Research on Basic Science from Shanghai Research Institute of Stomatology （S30206-KF09）.