Micro PET/CT在大鼠口腔黏膜癌前病变及口腔癌中的应用

Study of oral precancerous lesion and oral cancer by using micro PET/CT

目的:研究Micro PET/CT在大鼠口腔黏膜癌前病变及口腔癌中的应用价值。方法:采用4NQO饮水法诱导大鼠舌癌模型,将39只大鼠随机分为实验组及空白对照组。实验25～30周,进行Micro PET/CT扫描。扫描后行组织病理学检查。应用SAS6.0软件包,对正常组、癌前病变组及癌变组大鼠的SUVmax、T/NT(肌肉)、T/NT(脑组织)及T/NT(甲状腺)分别进行方差分析,并对T/NT值和病理学分级进行Spearman相关分析。结果:正常组、癌前病变组、癌变组大鼠舌根SUVmax无显著差异(P>0.05)。正常组与癌变组相比,T/NT(肌肉)及T/NT(脑组织)有显著差异(P<0.05),正常组与癌前病变组以及癌前病变组与癌变组,T/NT(肌肉)及T/NT(脑组织)无显著差异(P>0.05);T/NT(肌肉、脑组织)均与病理学分级呈正相关(P<0.05)。各组之间T/NT(甲状腺)差异无显著性(P>0.05),T/NT(甲状腺)与病理学分级无相关性(P>0.05)。结论:Mirco PET/CT作为一种无创检测技术,有助于对大鼠舌体组织癌变过程进行动态研究。T/NT(肌肉组织)、T/NT(脑组织)可作为大鼠舌体癌变过程中反映病损进展程度的有效指标。

PURPOSE： To study oral precancerous lesion and oral cancer by using micro PET/CT. METHODS： Thirty- nine SD rats were divided into experimental group and control group. 33 of them were raised with a 4-nitroquinoline-I - oxide （4NQO） solution with the concentration of 0.002% during the first 13 weeks, and then changed to normal water. The other 6 rats drank normal water all the time. During 25th to 30th week of the experiment, 2-Deoxy-2-[F-18]fluoro-D- glucose （FDG）-PET/CT was performed for these rats. One day after imaging, pathological examination was performed. SUVmax and T/NT were investigated according to pathological results. SAS6.0 software package was used for statistical analysis. RESULTS： There was no significant difference in SUVmax among the normal group, precancerous group and cancerous group （P〉0.05）. There was significant difference in T/NT （muscle, brain） between the normal group and the cancerous group （P〈0.05）. But there was no significant difference between the normal group and the precancerous group （P〉 0.05）; and no significant difference between the precancerous group and the cancerous group （P〉0.05）. The T/NT（muscle, brain） ratios increased along with the increase of the pathologic grade of the lesions. There was no significant difference in T/NT （thyroid） among the three groups and no correlation between the T/NT （thyroid） ratios and the pathologic grade. CONCLUSIONS： Micro PET/CT, as a non-invasive technology, may contribute to the dynamic studies of the process of carcinogenesis. T/NT （muscle, brain） ratios could show the degree of lesions of rat＇ s tongue during carcinogenesis. Supported by Research Fund of Science and Technology Commission of Shanghai Municipality（08DZ2271100） and Mirco PET/CT Fund of Interdisciplinary Research on Biomedicine and Engineering from Shanghai Jiao Tong University（YG08PETMS12）.