表皮生长因子对游离毛囊生长的影响

Effects of Epidermal Growth Factor on Human Hair Follicle Growth in vitro

目的 通过毛囊器官培养观察表皮生长因子（ EGF）对人头皮毛囊生长的影响。方法 用 Williams E无血清培养基进行人头皮毛囊游离培养，分为 2组， EGF组（ 43根）和对照组（ 43根）。结果 EGF 20μ g/L组在加药后 1～ 3 d毛干平均生长 0.22 mm/d，对照组平均生长 0.16 mm/d。

Objective To investigate the effects of epidermal growth factor (EGF) on hair follicle growth. Methods Cultured human hair follicles were divided into two groups: EGF- treated group (EGF 20 ng/mL) and control group. The length and morphology of follicles were measured by inverted microscope daily. 3H- leucine incorporation and histologic studies were applied. Results The rates of hair shaft and hair follicle elongation significantly increased in the EGF- treated group during day 1 to day 3 in comparison with the control group. Histologically, the cells of the lower outer root sheath were thickened and club hair- like structure was formed. 3H- leucine incorporation showed that the radioactivity in hair follicle epithelium was higher in EGF- treated group (370 c/min)than that in control group(271.14 c/min) (P