基于光电倍增管的化学发光免疫检测系统的研制

Development of Chemiluminescence Immune Detection System Based on Photomultiplier Tube

基于光电倍增管(PMT)设计了全自动化学发光免疫检测总体方案,以泌乳素(PRL)作为检测对象,标记酶采用辣根过氧化物酶(HRP),发光体为Pierce的鲁米诺(激发底物),对化学发光免疫检测系统PMT数据进行处理。通过检测5种不同浓度的HRP稀释液的发光数进行标定,后对数据进行线性化处理,得到PRL浓度与发光数的关系。两检测样本PRL分别取阳性范围浓度值(样本A)和阴性范围浓度值(样本B),同时将检测结果与LUMO与Liaison两种化学发光仪的检测结果进行比较。对于样本A,本系统、LUMO和Liaison的检测结果分别为51.724、46.198、43.792 ng/mL;对于样本B,本系统、LUMO和Liaison的检测结果分别为11.702、15.133、8.069 ng/mL。实验结果表明,所设计的全自动化学发光免疫检测系统可以确定被测样本的阴阳性,能够满足免疫分析应用需求。

In this work a full automatic chemiluminescence immune analyzer was designed and fabricated base on photomultiplier tube（PMT）.Experiments were conducted in which the prolactin（PRL） was taken as a detection object,the Horse Radish Peroxidase（HRP） served as Markey enzyme,and the Pierce Luminol（excitation substrate） was employed as a light resource.The PMT data obtained by the chemiluminescence immune detection system was processed,calibrated through the number of light emitting detection of five different consistency of PRL.The relationship between the consistency of PRL and the light-emitting was obtained.Samples in positive range of consistency（sample A） and negative range of consistency（sample B） and samples for LUMO and Liaison were measured.The results of sample A obtained from the present system,LUMO and Liaison were 51.724,46.198,43.792 ng/mL respectively,the data of sample B were 11.702,15.133,8.069 ng/mL respectively.It showed that the automatic chemiluminescence immune system can measure the negative or positive of samples and meet the application requirements of the immunoassay through the comparison with other chemiluminescence analyzers.