低强度脉冲超声波联合引导骨再生术对Beagle犬牙周骨开窗缺损修复效应的研究

Effect of Low-Intensity Pulsed Ultrasound Combined with Guided Bone Regeneration on Canine Periodontal Fenestration Defect in Beagle Dogs

本研究探讨了低强度脉冲超声波(LIPUS)联合引导骨再生术(GBR),对Beagle犬牙周骨开窗缺损的修复效应。构建5只Beagle犬尖牙颊侧根中1/3处5 mm×5 mm矩形牙周骨开窗缺损模型,将每只Beagle犬4颗尖牙随机分配到4个治疗组:LIPUS(90 mW/cm2,20 min/d)组、LIPUS(90 mW/cm2,20 min/d)+GBR组、GBR组、空白对照组,每组共计5颗实验牙。4周后处死Beagle犬,通过对实验区进行HE、Masson染色、Micro-CT检测以及对新生牙槽骨面积(NBA)及新生牙槽骨占初始缺损面积百分比(NBA%)的测量,比较各组牙周骨开窗缺损的组织学修复差异。Micro-CT观察显示,各组新生牙槽骨面积由大到小依次为LIPUS+GBR组、GBR组、LIPUS组、空白对照组;Masson染色显示,相比GBR组和空白对照组,LIPUS组和LIPUS+GBR组新生牙槽骨胶原成熟度较高;组织学测量各组NBA及NBA%,LIPUS组为(0.39±0.06)mm2,7.74%±1.09%;LIPUS+GBR组为(0.52±0.13)mm2,10.3%±2.22%;GBR组为(0.41±0.11)mm2,7.44%±2.20%;空白对照组为(0.24±0.04)mm2,4.64%±0.99%,各组间牙槽骨修复效果差异有统计学意义(P<0.05),新生牙槽骨量由大到小依次为LIPUS+GBR组、GBR组、LIPUS组、空白对照组。LIPUS辅助GBR治疗能够促进牙周骨开窗缺损的早期修复。

The aim of this work is to investigate the effect of combined application of low-intensity pulsed ultrasound（LIPUS） and guided bone regeneration（GBR） on canine periodontal fenestration defect in Beagle dogs.In the experiments,five Beagle dogs were used for establishing 5 mm×5 mm periodontal fenestration defect.Four canines in each dog were randomly divided into LIPUS（90 mW/cm^2,20 min/d）group,LIPUS（90 mW/cm^2,20 min/d）＋ GBR group,GBR group and Control group.Each group had 5 teeth.In order to compare the tissue repair among the groups,the specimens of periodontal fenestration were obtained for histological analysis（HE and Masson staining）,Micro-CT test and histometric analysis（the measurement of new bone area,NBA and its percentage in initial defect area,NBA%） after 4 weeks.Micro-CT test results showed that the area of new alveolar bone from large to small was LIPUS＋GBR group,GBR group,LIPUS group,and Control group.Histological analysis showed that the Masson staining of new bone in LIPUS and LIPUS＋GBR group was mainly red while GBR and control group was mainly blue.It suggested the new bone collagen in LIPUS group and LIPUS＋GBR group was more mature than in GBR group and control group.Histometric analysis showed that NBA and NBA% were 0.39 mm^2 and 7.74% in respectively LIPUS group;0.52 mm2 and 10.30% respectively in LIPUS＋GBR group;0.41 mm2 and 7.44% respectively in GBR group;0.24 mm2 and 4.64% respectively in Control group.Differences between groups were significant（P〈0.05）.It suggested the amount of new alveolar bone from large to small was LIPUS＋GBR group,GBR group,LIPUS group,and Control group.LIPUS combined with guided bone regeneration（GBR） can promote early repair of periodontal fenestration defect.