新型医用钛合金Ti-25Nb-10Ta-1Zr-0.2Fe的细胞毒性研究

Cytotoxicity of a new biomedical titanium alloy Ti-25Nb-10Ta-1Zr-0.2Fe

目的:比较传统医用钛合金Ti-6Al-4VELI(TC4ELI)和Ti-6Al-4V(TC4)、纯钛(Ti)以及新型低模量无Al,V钛合金Ti-25Nb-10Ta-1Zr-0.2Fe(TNTZ)4种金属材料的细胞毒性。方法:采用4种合金的浸提液培养L929细胞,另设空白对照组和阳性对照组。倒置相差显微镜下观察细胞形态及数量,MTT法测定各组细胞培养1,3,5 d的吸光度值,并计算细胞的相对增殖率,判断细胞毒性的级别。流式细胞仪测定不同浸提液处理的L929细胞不同增殖周期时相的DNA百分含量,计算S期细胞比率。结果:TNTZ处理组细胞3个时间点的相对增殖率分别为(93.7±0.8)%,(100.6±0.4)%,(106.4±0.3)%;培养1 d时细胞毒性分级为1级,培养3,5 d细胞毒性均为0级;与TC4ELI,TC4,Ti的细胞毒性分级相同。流式结果显示4种材料处理细胞后细胞S期细胞比率大小为Ti>TNTZ>TC4>空白对照>TC4ELI,但差异无统计学意义(P>0.05),均不抑制细胞增殖。结论:TNTZ合金不影响L929细胞的形态和增殖,与其他3种金属的细胞毒性级别相同,均为0级,符合临床应用标准,人体应用具有安全性。

Objective： To evaluate the cytotoxicity of a new type of titanium alloy Ti-25Nb-10Ta-1Zr-0.2Fe by studying the induced proliferation of L929 cells in contrast with other titania widely used in clinical practice.Methods： The cell line was treated with extracting liquid containing different concentrations of titanium alloys.The number and morphology of cells was observed under an inverted phase contrast microscope.MTT was used to measure the relative growth rate（RGR） and judge the cytotoxicity grade.Flow cytometry was used to observe cell cycle progression.Results： The RGR of TNTZ group cells at the 3 time points was（93.7±0.8）,（100.6±0.4）,and（106.4±0.3）;the cytotoxicity grade was 1,0 and 0 after treating for 1,3 and 5 days;with influence on neither the cell morphology nor the cell cycle.The flow cytometry showed that the sequence of S phase cells was TiTNTZTC4blank control TC4ELI,with no significant difference（P0.05）.None of the 4 materials inhibited the cell proliferation.Conclusion： The cell morphology and proliferation are not affected by TNTZ.The new titaniu alloys shows good cyto-compatibility.The cytotoxicity is grade 0,meeting the clinical application standard.