高糖对足细胞转分化和Smad7表达的影响

Effects of High Glucose on the Epithelial-to-mesenchymal Transition and the Expression of Smad7 in Cultured Podocytes

目的:通过观察高糖刺激下的足细胞表达synaptopodin、desmin和Smad7的变化,探讨高糖对足细胞的损伤机制。方法:将培养的小鼠足细胞分为正常糖组、高糖组、甘露醇组。倒置显微镜下观察足细胞的形态变化,采用RT-PCR和Western印迹技术分别检测synaptopodin、desmin、Smad7mRNA和蛋白的表达。结果:高糖刺激48h后的足细胞形态发生改变,并且synaptopodin、Smad7mRNA和蛋白表达较对照组减少,desmin mRNA和蛋白表达较对照组增加(P<0.01)。结论:高糖能够诱导足细胞发生转分化,并且这一作用可能与Samd7表达减少有关。

Objective ：To observe the changes of expression of synaptopodin ,desmin and Smad7 by high glucose exposure in cultured podocytes and to explore the possible injured mechanisms of high glucose. Methods：Mouse podocytes were cultured and di vided into normal glucose group（NG） ,high glucose group（HG）, mannitol group（MN）. The cell morphological changes were traced with inverted microscope. Real - time PCR and Western blot was applied respectively to detect the mRNA and protein expression lev- els of synaptopodin, desmin and Smad7. Results：The cells converted within 48h from typical arborized cells into cobblestone appear ance induced by high glucose. The cells significantly up - regulated the mRNA and protein expression of desmin, reduced the expres sion of synaptopodin and Smad7 induced by high glucose after 48 h （ vs. NG group, P 〈 0.01 ）. Conclusion： High glucose can induce epithelial -to -mesenchymal transition（EMT） in podocytes, and it maybe have a relationship with the reduced expression of Smad7.