脂质对肝星状细胞表达前胶原Ⅰ、Ⅲ mRNA的影响

Effect of lipids on Ⅰ and Ⅲ procollagen mRNA expression of hepatic stellate cells

目的探讨脂质（三酯酰甘油和极低密度脂蛋白）对 ＨＳＣ前胶原 Ⅰ、 ⅢｍＲＮＡ表达的影响。方法链霉蛋白酶和胶原酶原位灌流， Ｎｙｃｏｄｅｎｚ密度梯度离心分离大鼠 ＨＳＣ，分别用三酯酰甘油（２５ ｍｇ／Ｌ）和极低密度脂蛋白（２５ ｍｇ／Ｌ）共孵育 １０ ｄ后，提取 ＨＳＣ的 ＲＮＡ，用 Ｎｏｒｔｈｅｒｎ ｂｌｏｔ杂交观察三酯酰甘油和极低密度脂蛋白对ＨＳＣ前胶原Ⅰ、Ⅲ ｍＲＮＡ表达。结果 ＨＳＣ经三酯酰甘油和极低密度脂蛋白刺激后其前胶原Ⅰ、Ⅲ ｍＲＮＡ表达均明显增加。结论脂质可增加前胶原Ⅰ、Ⅲ ｍＲＮＡ表达，与脂肪肝肝纤维化的发生有关。

Objective To study the effect of lipids on Ⅰand Ⅲ procollagen mRNA expression of hepatic stellate cells(HSC). Methods HSC were isolated and cultured from the liver of Wistar rats by in situ perfusion with pronase and collagenase and the density gradient centrifugation with Nycodenz. HSC were incubated with triglyceride (25 mg/L) and very low-density lipid (VLDL, 25 mg/L) for 10 d, respectively. Ⅰand Ⅲ procollagen mRNA expression of HSC was de- tected by Northern blot hybridization. Results After HSC was stimulated with triglyceride and VLDL, the expression of Ⅰ and Ⅲ procollagen mRNA increased obviously. Conclusion Lipids may promote the expression of Ⅰand Ⅲ procollagen mRNA, and may be associated with fatty liver and hepatic fibrogenesis.