应用顺铂后肝癌细胞超微结构的研究

Ultrastructure study of hepatocellular carcinoma treated by diamminedichloroplatinum

目的了解经顺铂化疗后肝癌细胞的超微结构的变化。方法运用透射电镜观察经顺铂化疗后缩小切除的肝细胞癌的形态学特征的改变。结果细胞表面微绒毛消失，细胞接触松驰，细胞核缩小、碎裂、胞质空虚。内质网膜表面核糖体减少或缺少，糖原颗粒减少，线粒体空泡化或出现絮状物，并见凋亡小体。结论经顺铂化疗后的肝癌细胞的超微结构形态学改变符合程序性细胞死亡的过程。说明顺铂能诱导肝癌细胞发生凋亡。

Objective To study the changes of the ultrastructure of hepatocellular carcinoma(HCC) after being treated by diamminedichloroplatinum. Methods The changes of morphological feature of HCC treated by diam- minedichloroplatinum were observed with transmission electron microscope. Results The cells lost its specialized cell sur- face elements such as microvilli and cell-cell junctions. The nucleus chromatin condensed and segregated. The cytoplasm was hollow. The ribosomes on the endoplasmic reticulum reduced or lacked. The glycogen granules reduced. The mitochon- dria vacuolalized or produced flocculi, and the apoptotic bodies could be found. Conclusion The changes of the ultrastruc- ture of HCC treated by di amminedichloroplatinum correspond with the procedure of programmed cell death. Apoptosis of HCC cells can be induced by diamminedichloroplatinum.