人原发性肝癌细胞株受照射后凋亡与癌基因表达

Radiation-induced apoptosis and p53, bcl-2 gene expression products in QGY-7703 cell line in vitro

目的探讨人原发性肝癌细胞株受照射后凋亡与ｂｃｌ－２、ｐ５３基因表达产物关系。方法选择人原发性肝癌细胞株ＱＧＹ－７７０３，１８０ＫｖＸ线照射，流式细胞仪技术定量测定凋亡发生率，单克隆抗体免疫组化法测定受照射后ｂｃｌ－２、 ｐ５３表达产物， Ｓｏｎｙ Ｍｉａｓ－３００ Ｓｐａｔｏ图象分析仪分析结果。结果 ＱＧＹ－７７３０细胞株自发凋亡率为４．７９％，随照射后时间延长及照射剂量增加凋亡发生率增加。Ｂｃｌ－２、ｐ５３基因表达产物阳性率随照射后时间延长而增加，在照射后６ ｈ达峰值。结论照射后凋亡发生率与细胞群内在特性有关， ｐ５３及 ｂｃｌ－２基因表达产物阳性率峰值在照射后凋亡峰值之前数小时，提示它们共同参与了照射后调亡的调控。

Objective To investigate the dose response and time course of radiation-induced apoptosis and gene expression products of p53, bcl-2 genes in human hepatocarcinoma cell line QGY-7703 in vitro. Methods Apoptosis was induced by radiation and quantitated by Flow Cytometry. The oncoproteins of p53 and bcl-2 genes after radiation were de- tected immunocytochemically on cell cytospinned slides. The data were analyzed with Sony Mias-300 Spato Immage Analyzer. Results The percentage of apoptosis in unradiation QGY-7703 was 4.79%. After radiation, the apoptotic frac- tion increased with incubation time and radiation dose. Both gene expression products increased with incubation time and reached plateau at 6 h post-radiation. Conclusion After radiation, the response and time course of radiation-induced apoptosis in QGY-7703 cell line are similar to those of p53, bcl-2 oncoproteins. The later proceeds by the apoptosis peak about 6h. The results suggest that p53 and bcl-2 genes are the regulating factors partially in radiation-induced apoptosis of QGY-7703 cell line.