豆奶/“营养豆奶”对小鼠学习与记忆能力影响的研究

Effect of soybean milk/nutritional soybean milk on learning and memory ability of mice

探讨豆奶与铁、锌和／或维生素Ｃ强化豆奶（营养豆奶）改善学习记忆的功能。方法：选用初断乳小 鼠，喂饲豆奶或营养豆奶４周，进行水迷宫试验、跳台试验、避暗试验；并于试验不同阶段分别测定小鼠体重、血红 蛋白和血清铁。结果：①各试验组小鼠前２周体重增加值、血红蛋白增加值及血清铁值与对照组相比，均有增高趋 势，并以Ｆｅ＋Ｚｎ组及Ｆｅ＋Ｚｎ＋Ｖｃ组较明显；②水迷宫试验中，Ｆｅ＋Ｚｎ组及Ｆｅ＋Ｚｎ＋Ｖｃ组小鼠重测验时错误次数均显著低 于对照组（Ｐ＜０．０５， Ｐ＜０．０１）； Ｆｅ＋Ｚｎ＋Ｖｃ组训练及重测验时达标所需次数均显著少于对照组（Ｐ＜０．０５，Ｐ＜０．００２）； ③跳台试验中，各试验组重测验潜伏期均显著长于对照组（分别为Ｐ＜０．０５， ＜０．００１， Ｐ＜０．０１， Ｐ＜０．０５）；④避暗 试验中， Ｆｅ＋Ｚｎ组重测验时５ｍｉｎ内错误次数显著低于对照组（Ｐ＜０．０５）； Ｆｅ＋Ｚｎ＋Ｖｃ组训练及重测验潜伏期均显著短于 对照组 （Ｐ＜０．０５， Ｐ＜０．０１）．结论：豆奶、铁锌及铁锌维生素Ｃ强化豆奶均可促进初断乳小鼠的体格发育；铁锌及 铁锌维生素Ｃ强化豆奶还可能具有改善试验小鼠学习记忆能力的作用。

Objective: Research on the promoting effect of Soybean milk and for nutritional soybean milk on learning and memory ability. Methods: After feeding weanling mice with soybean milk or nutritional soybean milk for 4 weeks, we determined learning and memory ability and three common criteria of the mice, i.e. body weight, hemoglobin, serum iron. Result: ①The body weight, the value of hemoglobin, and serum iron of experimental groups were higher than those of control group, especially Fe+Zn and Fe+Zn+Vc groups; ② In maze experiment, the number of errors in training and retest of Fe+Zn and Fe+Zn+Vc groups were sigificantly lower than that of control group(P<0.05, p<0.01): ③ In jumping box experiment, the latent period (LP) of experimental groups were sigificantly longer than that of control group(P<0.05, P<0.001, P<0.01, P<0.05); ④ In dark escaping experiment, the number of errors in retest of Fe + Zn group was sigificantly lower than that of control group (P＜0.05); the training time in training and retest of Fe+Zn+Vc group was sigificantly shorter than that of control group .Conclusion: Soybean milk and nutritional soybean milk could promote physical development while nutritional soybean milk could improve learning and memory ability of experimental mice.