抗肿瘤抗生素云南霉素对细胞周期的作用及分子机理

Effect of antitumor antibiotic yunnanmycin on cell cycle and the molecular mechanism

目的 :研究具有新型结构的胞嘧啶核苷二肽类抗生素云南霉素的抗肿瘤作用的分子机理。方法 :应用细胞培养、流式细胞光度术、Westernblot分析等方法研究云南霉素对肿瘤细胞周期的影响及分子机理。结果 :云南霉素对多种肿瘤细胞 (HL 6 0 ,KB ,GLC)有G2 /M期阻滞、S期抑制作用 ,并伴有cyclinB1蛋白、P34 cdc2 蛋白、Mpm 2蛋白表达下降。结论 :云南霉素导致HL 6 0细胞G2 /M期阻滞的分子机理可能与降低cyclinB1、P34 cdc2和Mpm 2蛋白水平有关。

Objective: To explore the molecular mechanisms of antitumor effect of yunnanmycin which is a novel cytosine nucleoside antibiotic Methods: Cell culture, flow cytometry and Western blot analysis were employed toi ivestigate the effects of yunnanmycin on the cell cycle and related mechanisms Results: Arrest of G 2/M phase and inhibition of S phase were observed in HL 60, KB and GLC cells after treated with yunnanmycin Depressed expression of cyclin B1 protein, P34 cdc2 protein, Mpm 2 protein was found in the HL 60 cells treated with yunnanmycin Conclusions: The results suggest that the G 2/M arrest of HL 60 cells by yunnanmycin may be related to the altered low protein levels of cyclin B1, P34 cdc2 and Mpm 2