博安霉素诱导人鼻咽癌细胞凋亡作用

Boanmycin induces apoptosis in human nasopharyngeal cancer cells

目的 :探讨博安霉素 (博莱霉素A6 )抗人鼻咽癌作用的机理。方法 :应用荧光显微镜、流式细胞仪及琼脂糖凝胶电泳等方法检测博安霉素诱导人鼻咽癌SUNE 1细胞凋亡的作用。结果 :当博安霉素浓度为 5 0 μg/ml,作用于SUNE 1细胞 10小时 ,荧光显微镜下SUNE 1细胞出现明显的凋亡形态学改变 ;流式细胞仪出现亚G1峰 ,凋亡率为 46 .3% ;琼脂糖凝胶电泳出现典型的DNA梯状条带。结论 :博安霉素具有体外诱导鼻咽癌细胞凋亡的作用 ,而诱导凋亡为其抗肿瘤作用的机制之一。

Objective: To investigate the antitumor mechanisms of boanmycin (bleomycin A6) on human nasopharyngeal cancer. Method: Apoptosis induced by boanmycin in human nasopharyngeal cancer SUNE 1 cells was tested by fluorescent microscope, flowcytometry and DNA gel electro phoresis. Result: When incubated with boanmycin (50 μg/ml) for 10 hours, SUNE 1 cells showed morphological changes associated with the characters of apoptosis under fluorescent microscope. In flowcytometry analysis sub G1 peak appeared in DNA frequency distribution histograms. Typical DNA ladder was found during gel electrophoresis. Conclusion: Boanmycin induces apoptosis in SUNE 1 cells in vitro, which may be one of its antitumor mechanisms of action against nasopharyngeal cancer.