反义bcl-2基因转染对单核白血病细胞存活及化疗耐受能力的影响

Influence of antisense bcl-2 gene on survival of mononuclear leukemia cell U937 and the sensitivity of the cell to chemotherapeutic drugs

目的 :探讨bcl 2反义核酸在单核白血病细胞系U937细胞中的作用 ,以丰富bcl 2及其反义核酸调控肿瘤细胞凋亡的资料。方法 :将反义bcl 2基因表达载体 pLXSN/as bcl 2转染于U937细胞 ,建立U937/as bcl 2细胞模型 ,利用流式细胞技术检测模型细胞bcl 2的表达水平 ;通过细胞计数观察模型细胞在正常培养条件下和有化疗药物Ara C或三尖杉酯碱存在时的生长和存活特性变化。结果 :①模型细胞的bcl 2蛋白表达水平明显下降。②模型细胞在正常培养条件下的生长和存活表型无明显改变。③在Ara C或三尖杉酯碱存在的情况下 ,模型细胞的存活率较对照细胞者明显下降。结论 :①单独反义bcl 2基因转染可明显抑制U937细胞bcl 2的表达 ,但对细胞在正常条件下的生长和存活表型无明显影响。②反义bcl 2基因转染可增加U937细胞对化疗药物的敏感性。

Objective:To enrich the data of tumor cell apoptosis regulated by bcl 2 and antisense bcl 2 through studying the role of antisense bcl 2 in mononuclear leukemia cell line U937 Methods:A U937 cell model (U937/as bcl 2) was established by transferring the antisense bcl 2 expression vector pLXSN/as bc1 2 into U937 cell bcl 2 expression in the model cell was detected by fluorescence activated cell sorter The model cell growth and survival were analyzed by counting the numbers of total and survival cells in normal culture condition or in the presence of Ara C or harringtonine Results:①bcl 2 expression in the model cell significantly decreased ②The model cell had a normal growth and survival in normal culture condition ③The percentage of the survival model cells significantly decreased in the presence of Ara C or harringtonine Conclusions:①Antisense bcl 2 gene transfer alone can significantly suppress bcl expression in U937 cells, but it had no obvious effect on the cell growth and survival in normal culture condition ②Antisense bcl 2 gene transfer can increase the sensitivity of U937 cell to chemotherapeutic drugs