多药耐药基因mdr-1在肝门部胆管癌组织中的表达及其意义

The expression of mdr-1 mRNA in hilar cholangiocarcinoma

目的 :检测肝门部胆管癌新鲜组织中多药耐药基因 (mdr - 1)mRNA表达 ,探讨其与肝门部胆管癌临床病理间的关系。方法 :应用逆转录多聚酶链反应 (RT PCR)对 2 6例术前未作治疗的肝门部胆管癌 ,12例正常胆管组织的mdr 1mRNA表达进行了检测 ,并与癌组织的分化程度、病理类型、部位、浸润转移作对比研究。结果 :2 6例肝门部胆管癌组织中mdr 1mRNA阳性表达率为 6 5 4% (17/ 2 6 ) ,正常胆管组织表达率为 8 3% (1/ 12 ) ,二者差异有显著性 (P <0 0 1)。mdr 1mRNA阳性表达与肝门部胆管癌的分化程度、发生部位、病理类型、浸润转移无关 (P >0 0 5 )。结论 :肝门部胆管癌组织中mdr 1mRNA的高表达可作为肝门部胆管癌化疗耐药的指标。

Objectives:To detect the levels of mdr l gene experession in fresh untreated hilar cholangiocarcinoma tissues, and to investigate the correlation of mdr 1 expression with current prognostic indicators of clinicopathology Method:mdr 1 mRNA expression of 26 cases untreated hilar cholangicarcinoma and 12 cases normal bile duct tissues were examined using reverse transcriptase polymerase chain reaction (RT PCR) Results:mdr 1 mRNA expression in tumor tissue was found in 65 4%(17/26) of the patients, whereas, 8 3%(1/12) was seen in normal bile duct (P< 0 01) No correlation with differentiation, invassiveness, metastasis and staging, clinical typing, pathological type was found (P >0 05) Conclusion:Higher expression of mdr 1 mRNA in hilar cholangiocarcinoma tissue might be served as an indicator for drug resistance in chemotherapy